Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae)

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	Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae)
	Liu, Li-Qin 1,2; Gu, Zhi-Jian 1
刊名	PLANT SCIENCE
	2011-03-01
卷号	180 期号:3 页码:554-559
关键词	Camellia reticulata C. pitardii C. saluenensis Genomic in situ hybridization (GISH) Genome donors
ISSN号	0168-9452
通讯作者	Liu, LQ (reprint author), Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
英文摘要	Camellia reticulata (Theaceae genus Camellia) is a world-famous, ornamental flowering plant. More interestingly, it has a polyploid series varying from 2n = 2x = 30, 2n = 4x = 60 to 2n = 6x = 90, with a basic chromosome number of x = 15. The hypothetic allopolyploid origin and parental genomes of these polyploid types remains unknown. Genomic in situ hybridization (GISH) was used to study the genome organization and evolution of C. reticulata. Total genomic DNA from closely-related diploid species (C pitardii and C saluenensis), with the chromosome number 2n = 2x = 30, were labeled and hybridized in the presence of blocking DNA onto metaphase spreads of C reticulata. The C pitartii probe painted part of the tetraploid and hexaploid C reticulata genomes, whereas the C saluenensis probe delineated part of the hexaploid C reticulata genome. The results provide compelling evidence for the allopolyploid origin of C. reticultata genomes and demonstrate that 1) the diploid C. reticultata, C. pitardii and C. saluenensis are the progenitors of polyploid C reticultata, 2) hybridization between diploid C reticultata and diploid C pitardii gave birth to allotetraploid C. reticulata, and 3) subsequent hybridization between allotetraploid C. reticulata and diploid C. saluenensis formed the allohexaploid C. reticulata. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Plant Sciences
类目[WOS]	Biochemistry & Molecular Biology ; Plant Sciences
研究领域[WOS]	Biochemistry & Molecular Biology ; Plant Sciences
关键词[WOS]	HYBRID ; GISH ; FISH
收录类别	SCI
资助信息	National Natural Science Foundation of China[30570114]
语种	英语
WOS记录号	WOS:000287114500019
公开日期	2012-04-10
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/5307]
专题	昆明植物研究所_资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Li-Qin,Gu, Zhi-Jian. Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae)[J]. PLANT SCIENCE,2011,180(3):554-559.
APA	Liu, Li-Qin,&Gu, Zhi-Jian.(2011).Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae).PLANT SCIENCE,180(3),554-559.
MLA	Liu, Li-Qin,et al."Genomic in situ hybridization identifies genome donors of Camellia reticulata (Theaceae)".PLANT SCIENCE 180.3(2011):554-559.