Molecular Authentication of the Ethnomedicinal Plant Sabia parviflora and Its Adulterants by DNA Barcoding Technique

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 资源植物与生物技术所级重点实验室

	Molecular Authentication of the Ethnomedicinal Plant Sabia parviflora and Its Adulterants by DNA Barcoding Technique
	Sui, Xue-yi 2; Huang, Yuan ; Tan, Ying 3; Guo, Yu 2; Long, Chun-lin 1,4
刊名	PLANTA MEDICA
	2011-03-01
卷号	77 期号:5 页码:492-496
关键词	Sabia parviflora Sabiaceae DNA barcoding ethnomedicinal plant authentication
ISSN号	0032-0943
通讯作者	Long, CL (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, 132 Lanhei Rd, Kunming 650204, Yunnan, Peoples R China
英文摘要	Sabia parviflora Wall. ex Roxb. is a traditional herb widely used by Chinese people, especially by the Buyi ethnic group which resides in Guizhou and Yunnan provinces. According to the Chinese Ethnic Pharmacopeia, the species is commonly used for soothing the liver and for the treatment of icteric hepatitis, hemostasis, and inflammation. However, due to the similar morphological characters of Sabia species and higher market demands, there are many substitutes and adulterants of S. parviflora. In this study, the differential identification of 6 Sabia species and 7 adulterants were investigated through DNA sequence analysis of three candidate DNA barcodes (trnH-psbA, rbcL-a, matK). Based on sequence alignments, we concluded that not only the trnH-psbA spacer sequence can distinguish S. parviflora from other Sabia species, but the matK + rbcL-a sequences also can differentiate it from the substitutes and adulterants. The classification tree of all samples based on rbcL-a sequences indicated that the rbcL region can identify samples into a family/genus level. Our results suggest that the three candidate barcodes can be used for the identification of S. parviflora and to distinguish it from common substitutes or adulterants.
学科主题	Plant Sciences; Pharmacology & Pharmacy
类目[WOS]	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]	Plant Sciences ; Pharmacology & Pharmacy
关键词[WOS]	LAND PLANTS
收录类别	SCI
资助信息	Ministry of Education of China[MUC 98503-001006, B08044]; Chinese Academy of Sciences[O92441112F, KSCX2-YW-Z-0925]; National Science Foundation of China[31070288]; Japan Society for the Promotion of Science[JSPS/AP/109080]; Ministry of Science and Technology of China[2008FY110400-2-2]
语种	英语
WOS记录号	WOS:000288847600015
公开日期	2012-04-10
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/5281]
专题	昆明植物研究所_资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650204, Yunnan, Peoples R China 2.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 4.Minzu Univ China, Coll Life & Environm Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Sui, Xue-yi,Huang, Yuan,Tan, Ying,et al. Molecular Authentication of the Ethnomedicinal Plant Sabia parviflora and Its Adulterants by DNA Barcoding Technique[J]. PLANTA MEDICA,2011,77(5):492-496.
APA	Sui, Xue-yi,Huang, Yuan,Tan, Ying,Guo, Yu,&Long, Chun-lin.(2011).Molecular Authentication of the Ethnomedicinal Plant Sabia parviflora and Its Adulterants by DNA Barcoding Technique.PLANTA MEDICA,77(5),492-496.
MLA	Sui, Xue-yi,et al."Molecular Authentication of the Ethnomedicinal Plant Sabia parviflora and Its Adulterants by DNA Barcoding Technique".PLANTA MEDICA 77.5(2011):492-496.