Synthesis and Characterization of the Hexachlorotriphosphaxene Cross-linked Oligo(ethyleneimine) as Gene Carrier

	Synthesis and Characterization of the Hexachlorotriphosphaxene Cross-linked Oligo(ethyleneimine) as Gene Carrier
	Li FF ; Tian HY ; Chen L ; Xia JL ; Chen XS ; Jing XB
刊名	chemical journal of chinese universities-chinese
	2010
卷号	31 期号:9 页码:1896-1900
关键词	DELIVERY-SYSTEMS POLYPHOSPHAZENES POLYETHYLENIMINE POLYMERS COPOLYMER CANCER CELLS
ISSN号	0251-0790
通讯作者	chen xs
中文摘要	hyperbranched oligo( ethyleneimine) with molecular weight of 600 was carried out reaction with hexachlorotriphosphazene in anhydrous chloroform to synthesize a degradable and cross-linked polymer as a non-viral gene carrier. the h-1 nmr spectra confirmed the formation of the polymer and its average molecular weight was obtained by gpc with hac/naac buffering solution of ph = 4. 4 as fluent phase. the polymer compressed dna into stable complexes in diameter of about 150 nm and zeta potential of 30-40 mv measured by dynamic light scattering. besides, when the mass ratio of polymer and dna was 0. 4, the plasmids were fully retarded in 1% agarose gel by gel retardation electrophoresis. the cytotoxicity and transfection efficiency were characterized by hela cells in vitro. it was indicated that the best transfection efficiency of polymer with lower cytotoxicity was three times higher than that of pei-25k. therefore, the synthesized polymer may have good potential applications as non-viral gene carriers.
收录类别	SCI收录期刊论文
语种	中文
WOS记录号	WOS:000282379300037
公开日期	2012-04-25
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/43429]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li FF,Tian HY,Chen L,et al. Synthesis and Characterization of the Hexachlorotriphosphaxene Cross-linked Oligo(ethyleneimine) as Gene Carrier[J]. chemical journal of chinese universities-chinese,2010,31(9):1896-1900.
APA	Li FF,Tian HY,Chen L,Xia JL,Chen XS,&Jing XB.(2010).Synthesis and Characterization of the Hexachlorotriphosphaxene Cross-linked Oligo(ethyleneimine) as Gene Carrier.chemical journal of chinese universities-chinese,31(9),1896-1900.
MLA	Li FF,et al."Synthesis and Characterization of the Hexachlorotriphosphaxene Cross-linked Oligo(ethyleneimine) as Gene Carrier".chemical journal of chinese universities-chinese 31.9(2010):1896-1900.