Studying copper(II) ion induced interactions of beta-amyloid peptides within living cells by gold nanoparticle probes

	Studying copper(II) ion induced interactions of beta-amyloid peptides within living cells by gold nanoparticle probes
	Wang CK ; Wang JN ; Liu DJ ; Wang ZX
刊名	analytical methods
	2010
卷号	2 期号:10 页码:1467-1471
关键词	ALZHEIMERS-DISEASE METAL-BINDING AGGREGATION HYPOTHESIS AMYLOID-BETA(1-16) ENZYME CYCLE
ISSN号	1759-9660
通讯作者	wang zx
中文摘要	in this paper, a kind of gold nanoparticle (gnp)-based colorimetric assay was developed for studying copper(ii) ion (cu2+) induced interaction of beta-amyloid (ab) peptide with living cells. in the presence of cu2+, biotin modified beta-amyloid peptides (a beta(1-16)(biotin)) enable to attach on the shg-44 (human glioma cell) cellular surface which results in significant color change of the cells when the indicators, streptavidin conjugated gnps were introduced into the reaction mixture. we also demonstrate the assay ability to screen a beta aggregation inhibitors because the aggregating/binding process can be easily reversed by adding chelators (e.g., edta, egta, histidine and clioquinol) of cu2+ to the system. in addition, a time-dependent interaction of shg-44 cells with cu2+ and a beta(1-16)(biotin) has been observed that may suggest different expression levels of a beta related proteins in various cell cycles.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000283959600007
公开日期	2012-04-25
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/43311]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang CK,Wang JN,Liu DJ,et al. Studying copper(II) ion induced interactions of beta-amyloid peptides within living cells by gold nanoparticle probes[J]. analytical methods,2010,2(10):1467-1471.
APA	Wang CK,Wang JN,Liu DJ,&Wang ZX.(2010).Studying copper(II) ion induced interactions of beta-amyloid peptides within living cells by gold nanoparticle probes.analytical methods,2(10),1467-1471.
MLA	Wang CK,et al."Studying copper(II) ion induced interactions of beta-amyloid peptides within living cells by gold nanoparticle probes".analytical methods 2.10(2010):1467-1471.