A Prefractionation Method Can Separate Proteomic Proteins into Multigroups by One-step Extraction

	A Prefractionation Method Can Separate Proteomic Proteins into Multigroups by One-step Extraction
	Bai HX ; Liu XH ; Yang F ; Yang XR ; Wang EK
刊名	journal of the chinese chemical society
	2010
卷号	57 期号:5a 页码:1007-1014
关键词	AQUEOUS 2-PHASE SYSTEMS MASS-SPECTROMETRY IDENTIFICATION HUMAN PLASMA PROTEOME MEMBRANE-PROTEINS SAMPLE PREFRACTIONATION AMMONIUM-SULFATE LOW-ABUNDANCE ELECTROPHORESIS COMPLEX MODEL
ISSN号	0009-4536
通讯作者	bai hx
中文摘要	a variety of prefractionation methods for proteome analysis had been developed, but some of these had defects such as requirements sophisticated apparatus, expensive reagents, too long experimental time or complicated operation. in this work, a simple, rapid and low-cost prefractionation method for proteome analysis was developed based on an aqueous two-phase system (atps), which used simple and inexpensive components in place of the polymers, could pre-separate proteomic proteins into multigroups by one-step extraction according proteins' difference in solubility. the atps consisted of isopropanol, ammonium sulfate and water. the highlight attractiveness of this method included simplicity, rapidity, low cost and the ability of concentrating target proteins. attractiveness of this method also included biocompability, amenability to linear scale-up, potential for continuous operation, and being easy or no need for separating the target proteins from phase-forming reagents before gel electrophoresis analysis. it was the first time that the atps composed of isopropanol, ammonium sulfate and water was used in studying the prefractionation method for proteome analysis. thus, this method had great significance in proteome analysis and methodology.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000285525900007
公开日期	2012-04-25
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/43223]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Bai HX,Liu XH,Yang F,et al. A Prefractionation Method Can Separate Proteomic Proteins into Multigroups by One-step Extraction[J]. journal of the chinese chemical society,2010,57(5a):1007-1014.
APA	Bai HX,Liu XH,Yang F,Yang XR,&Wang EK.(2010).A Prefractionation Method Can Separate Proteomic Proteins into Multigroups by One-step Extraction.journal of the chinese chemical society,57(5a),1007-1014.
MLA	Bai HX,et al."A Prefractionation Method Can Separate Proteomic Proteins into Multigroups by One-step Extraction".journal of the chinese chemical society 57.5a(2010):1007-1014.