The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 淡水生态学研究中心 > 期刊论文

	The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR
	Zhao, Sujuan 1; Xie, Ping 1; Li, Guangyu 1,2; Jun, Chen 1; Cai, Yan 1; Xiong, Qian 1; Zhao, Yanyan 1
刊名	PROTEOMICS
	2012
卷号	12 期号:2 页码:300-312
关键词	2-DE Animal proteomics MC-RR Reproductive toxicity Testis Ultrastructure
ISSN号	1615-9853
通讯作者	Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China
中文摘要	Microcystin-RR (MC-RR) is a commonly encountered cyanotoxin and receives increasing attention due to the risk of its bioaccumulation in aquatic animals like fish. This study investigated the protein profiles of zebrafish (Danio rerio) testes after intraperitoneal injection (i.p.) with 0.5 LD50 (2000 mu g/kg). MC-RR caused a noticeable damage to testicular ultrastructure, showing widened intercellular junction, distention of mitochondria. The testes showed a rapid response of its defense systems to the oxidative stress caused by MC-RR. This is the first to use a proteomic approach to obtain an overview of the effects of MC-RR on the testes of zebrafish. The proteomic results revealed that toxin exposure remarkably altered the abundance of 24 proteins that were involved in cytoskeleton assembly, oxidative stress, glycolysis metabolism, calcium ion binding and other biological functions. In conclusion, MC-RR damaged the testes and was toxic to the reproductive system of male zebrafish mainly through causing oxidative stress.
英文摘要	Microcystin-RR (MC-RR) is a commonly encountered cyanotoxin and receives increasing attention due to the risk of its bioaccumulation in aquatic animals like fish. This study investigated the protein profiles of zebrafish (Danio rerio) testes after intraperitoneal injection (i.p.) with 0.5 LD50 (2000 mu g/kg). MC-RR caused a noticeable damage to testicular ultrastructure, showing widened intercellular junction, distention of mitochondria. The testes showed a rapid response of its defense systems to the oxidative stress caused by MC-RR. This is the first to use a proteomic approach to obtain an overview of the effects of MC-RR on the testes of zebrafish. The proteomic results revealed that toxin exposure remarkably altered the abundance of 24 proteins that were involved in cytoskeleton assembly, oxidative stress, glycolysis metabolism, calcium ion binding and other biological functions. In conclusion, MC-RR damaged the testes and was toxic to the reproductive system of male zebrafish mainly through causing oxidative stress.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	CARP CYPRINUS-CARPIO ; ALDEHYDE DEHYDROGENASE ; INTERMEDIATE-FILAMENTS ; REPRODUCTIVE-SYSTEM ; OXIDATIVE STRESS ; HUMAN HEALTH ; LR ; LIVER ; GLUTATHIONE ; TOXICITY
收录类别	SCI
资助信息	National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
语种	英语
WOS记录号	WOS:000299153500014
公开日期	2012-04-01
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16717]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Sujuan,Xie, Ping,Li, Guangyu,et al. The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR[J]. PROTEOMICS,2012,12(2):300-312.
APA	Zhao, Sujuan.,Xie, Ping.,Li, Guangyu.,Jun, Chen.,Cai, Yan.,...&Zhao, Yanyan.(2012).The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR.PROTEOMICS,12(2),300-312.
MLA	Zhao, Sujuan,et al."The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR".PROTEOMICS 12.2(2012):300-312.