Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States

	Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States
	Dai GL(戴国亮); Sazaki Gen; Matsui Takuro; Tsukamoto Katsuo; Nakajima Kazuo; Kang Q(康琦); Hu WR(胡文瑞)
刊名	Crystal Growth & Design
	2011
通讯作者邮箱	sazaki@lowtem.hokudai.ac.jp
卷号	11 期号:1 页码:88-92
关键词	Reflection Fluorescence Microscopy Single-Protein Molecules Crystals Adsorption Diffusion Interface Surface
ISSN号	1528-7483
通讯作者	Sazaki, G (reprint author), Hokkaido Univ, Inst Low Temp Sci, N19-W8 Kita Ku, Sapporo, Hokkaido 0600819, Japan
产权排序	[Dai, Guoliang; Kang, Qi; Hu, Wenrui] Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China; [Sazaki, Gen; Matsui, Takuro; Nakajima, Kazuo] Tohoku Univ, Inst Mat Res, Sendai, Miyagi 9808577, Japan; [Matsui, Takuro] Merck Ltd Japan, New Technol Lab, Kanagawa 2430303, Japan; [Tsukamoto, Katsuo] Tohoku Univ, Grad Sch Sci, Sendai, Miyagi 9808577, Japan; [Dai, Guoliang; Sazaki, Gen; Matsui, Takuro] Tohoku Univ, Interdisciplinary Res Ctr, Sendai, Miyagi 9808577, Japan
合作状况	国际
中文摘要	The adsorption/desorption behavior of mobile solute molecules at a solution-crystal interface has been explored using crystals of model protein hen egg-white lysozyme (HEWL) and fluorescent-labeled HEWL (F-HEWL) molecules. We have tracked the transient processes occurring during adsorption/desorption of identical F-HEWL molecules on a tetragonal HEWL crystal surface by single-molecule visualization using a total internal reflection fluorescent microscope and pulsed discontinuous laser illumination. We found an induction period (similar to 70 min) after which the number density of F-HEWL molecules adsorbed mainly on steps increased linearly with the adsorption time. We show direct evidence that the residence time of molecules on the crystal surface gradually increases during the transition process from a solute species to the crystal after successive multistep processes.
学科主题	Chemistry; Crystallography; Materials Science
分类号	一类
类目[WOS]	Chemistry, Multidisciplinary ; Crystallography ; Materials Science, Multidisciplinary
研究领域[WOS]	Chemistry ; Crystallography ; Materials Science
关键词[WOS]	REFLECTION FLUORESCENCE MICROSCOPY ; SINGLE-PROTEIN MOLECULES ; CRYSTALS ; ADSORPTION ; DIFFUSION ; INTERFACE ; SURFACE
收录类别	SCI ; EI
资助信息	The authors thank H. Higuchi (The University of Tokyo) for valuable discussions, and they acknowledge grants from the National Basic Research Program of China (973 Program) (G.D.: No. 2011CB710901), the Knowledge Innovation Program of the Chinese Academy of Sciences (G.D.: No. KJCX2-YW-L08), and the National Nature Science Foundation of China (G.D.: No. 20603043), and Grants-in-Aid (G.S.: Nos. 17034007 and 18360003) from the Scientific Research of the Ministry of Education, Science and Culture, Japan. One of the authors (G.D.) acknowledges a grant from Tohoku University as a visiting professor. This work was carried out as a part of "Project Research B" at the Center for Interdisciplinary Research, Tohoku University, as well as the "Ground-based Research Announcement for Space Utilization" of the Japan Space Forum.
原文出处	http://dx.doi.org/10.1021/cg100869r
语种	英语
WOS记录号	WOS:000285735100015
公开日期	2012-04-01
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/45110]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Dai GL,Sazaki Gen,Matsui Takuro,et al. Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States[J]. Crystal Growth & Design,2011,11(1):88-92.
APA	戴国亮.,Sazaki Gen.,Matsui Takuro.,Tsukamoto Katsuo.,Nakajima Kazuo.,...&胡文瑞.(2011).Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States.Crystal Growth & Design,11(1),88-92.
MLA	戴国亮,et al."Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States".Crystal Growth & Design 11.1(2011):88-92.