Fluid Dynamics Analysis of a Novel Micropatterned Cell Bioreactor

	Fluid Dynamics Analysis of a Novel Micropatterned Cell Bioreactor
	Cui YH; Huo B(霍波); Sun SJ(孙树津); Yang F(杨帆); Gao YX(高宇欣); Pan J; Long M(龙勉)
刊名	Annals of Biomedical Engineering
	2011
通讯作者邮箱	mlong@imech.ac.cn
卷号	39 期号:5 页码:1592-1605
关键词	Fluid Dynamics Cell Bioreactor Micropattern Steady Shear-Stress Endothelial-Cells Flow Mechanotransduction Environment Prediction Scaffolds Adhesion Chamber Systems
ISSN号	0090-6964
通讯作者	Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
产权排序	[Huo, Bo; Sun, Shujin; Yang, Fan; Gao, Yuxin; Long, Mian] Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China; [Cui, Yuhong] Tianjin Univ, Dept Mech, Tianjin 300072, Peoples R China; [Huo, Bo; Sun, Shujin; Yang, Fan; Gao, Yuxin; Long, Mian] Natl Micrograv Lab, Beijing 100190, Peoples R China; [Huo, Bo; Sun, Shujin; Yang, Fan; Gao, Yuxin; Long, Mian] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China; [Pan, Jun] Chongqing Univ, Bioengn Coll, Chongqing 400030, Peoples R China
合作状况	国内
中文摘要	Although flow-based bioreactor has been widely used to provide sufficient mass transportation and nutrient supply for cell proliferation, differentiation, and apoptosis, the underlying mechanism of cell responses to applied flow at single cell level remains unclear. This study has developed a novel bioreactor that combines flow bioreactor with microfabrication technique to isolate individual cells onto micropatterned substrate. A mechanical model has also been developed to quantify the flow field or the microenvironment around the single cell; flow dynamics has been analyzed on five geometrically different patterns of circle-, cube-, 1:2 ellipse-, 1:3 ellipse-, and rectangle-shaped "virtual cells." The results of this study have demonstrated that the flow field is highly pattern dependent, and all the hydrodynamic development length, cell spacing, and orientation of inlet velocity vector are crucial for maintaining a fully developed flow. This study has provided a theoretical basis for optimizing the design of micropatterned flow bioreactor and a novel approach to understand the cell mechanotransduction and cell-surface interaction at single cell level.
学科主题	Engineering
分类号	二类/Q2
类目[WOS]	Engineering, Biomedical
研究领域[WOS]	Engineering
关键词[WOS]	STEADY SHEAR-STRESS ; ENDOTHELIAL-CELLS ; FLOW ; MECHANOTRANSDUCTION ; ENVIRONMENT ; PREDICTION ; SCAFFOLDS ; ADHESION ; CHAMBER ; SYSTEMS
收录类别	SCI ; EI
资助信息	The authors are grateful to Xin Wang, Yunfeng Wu, and Yabin Zhai for computational assistance. This study was supported by the following grants: the Natural Science Foundation of China, #30730032 and #30870606; Knowledge Innovation Program of CAS, #KJCX2-YW-L08; the National Key Basic Research Foundation of China, #2006CB910303; and the National High Technology Research and Development Program of China, #2007AA02Z306.
原文出处	http://dx.doi.org/10.1007/s10439-011-0250-4
语种	英语
WOS记录号	WOS:000289243100020
公开日期	2012-04-01
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/45086]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Cui YH,Huo B,Sun SJ,et al. Fluid Dynamics Analysis of a Novel Micropatterned Cell Bioreactor[J]. Annals of Biomedical Engineering,2011,39(5):1592-1605.
APA	Cui YH.,霍波.,孙树津.,杨帆.,高宇欣.,...&龙勉.(2011).Fluid Dynamics Analysis of a Novel Micropatterned Cell Bioreactor.Annals of Biomedical Engineering,39(5),1592-1605.
MLA	Cui YH,et al."Fluid Dynamics Analysis of a Novel Micropatterned Cell Bioreactor".Annals of Biomedical Engineering 39.5(2011):1592-1605.