A Modified Enrichment Method to Construct Microsatellite Libraryfrom Plateau Pika Genome (Ochotona curzoniae)

	A Modified Enrichment Method to Construct Microsatellite Libraryfrom Plateau Pika Genome (Ochotona curzoniae)
	Geng Jianing ; Li Kexin ; Zhang Yanming ; Hu Songnian
刊名	genomics, proteomics & bioinformatics
	2010
卷号	8 期号:1 页码:72-76
关键词	microsatellite enriched library magnetic bead ultrasonication
中文摘要	a microsatellite-enriched library of plateau pika (ochotona curzoniae) was constructed according to the strongaffinity between biotin and streptavidin. firstly, genomic dna was fragmented by ultrasonication, which is a majorimprovement over traditional methods. linker-ligated dna fragments were hybridized with biotinylated microsatelliteprobes, and then were subjected to streptavidin-coated magnetic beads. pcr amplificationwasperformedto obtain double-stranded dna fragments containing microsatellites. ligation and transformation werecarried out by using the pgem-t vector system i and escherichia coli dh10b competent cells. sequencing resultsshowed that 80.2% of clones contained microsatellite repeatmotif. several modifications make this protocoltime-efficient and technically easier than the traditional ones; particularly,composition and relative abundance ofmicrosatellite repeats in plateau pika genome were truly represented through the optimized pcr conditions. thismethod has also been successfully applied to construct microsatellite-enriched genomic libraries of chinese hamster(cricetulus griseus) and small abalone [haliotis diversicolor (reeve)] with high rates of positive clones,demonstrating its feasibility and stability.
收录类别	CSCD
公开日期	2011-12-09
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn//handle/363003/1492]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Geng Jianing,Li Kexin,Zhang Yanming,et al. A Modified Enrichment Method to Construct Microsatellite Libraryfrom Plateau Pika Genome (Ochotona curzoniae)[J]. genomics, proteomics & bioinformatics,2010,8(1):72-76.
APA	Geng Jianing,Li Kexin,Zhang Yanming,&Hu Songnian.(2010).A Modified Enrichment Method to Construct Microsatellite Libraryfrom Plateau Pika Genome (Ochotona curzoniae).genomics, proteomics & bioinformatics,8(1),72-76.
MLA	Geng Jianing,et al."A Modified Enrichment Method to Construct Microsatellite Libraryfrom Plateau Pika Genome (Ochotona curzoniae)".genomics, proteomics & bioinformatics 8.1(2010):72-76.