A GC/MS method for the assessment of N-15 and C-13 incorporation into soil amino acid enantiomers

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	A GC/MS method for the assessment of N-15 and C-13 incorporation into soil amino acid enantiomers
	Zhang, XD ; He, HB ; Amelung, W
刊名	SOIL BIOLOGY & BIOCHEMISTRY
	2007
卷号	39 期号:11 页码:2785-2796
关键词	amino acid enantiomer C-13 N-15 incubation GC/MS atom percentage excess (APE)
ISSN号	0038-0717
通讯作者	Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
中文摘要	Identifying the transformation process of amino acid enantionters was essential to probe into the fate, turnover and aging of soil nitrogen due to their important roles in the biogeochemical cycling. If this can be achieved by differentiating between the newly biosynthesized and the inherent compounds in soil, then the isotope tracer method can be considered most valid. We thereby developed a gas chromatography/mass spectrometry (GC/MS) method to trace the N-15 or C-13 isotope incorporation into soil amino acid enantiomers after being incubated with (NH4+)-N-15 or U-C-13-glucose substrates. The most significant fragments (F) as well as the related minor ions were monitored by the full scan mode and the isotope enrichment in amino acids was estimated by calculating the atom percentage excess (APE). (NH4+)-N-15 incorporation was evaluated according to the relative abundance increase of m/z F + I to F for neutral and acidic amino acids and F + 2 to F (mass 439) for lysine. The assessment of C-13 enrichment in soil amino acids was more complicated than that of N-15 due to multi-carbon atoms in amino acid molecules. The abundance ratio increment of m/z F + n to F (n is the original skeleton carbon number in each fragment) indicated the direct conversion from the added glucose to amino acids, but the total isotope incorporation from the added C-13 can only be calculated according to all target isotope fragments, i.e. the abundance ratio increment summation from m/z (Fa + 1) through m/z (Fa + T) represented the total incorporation of the added C-13 (Fa is the fragment containing all original skeleton carbons and T is the carbon number in the amino acid molecule). This method has a great advantage especially for the evaluation of high-abundance isotope enrichment in organic compounds compared with GC/C/IRMS. And in principle, this technique is also valid for amino acids besides enantiomers if stereoisomers are not concerned. Our assessment approach could shine a light on investigating the biochemical mechanism of microbial transformation of N and C in soils of terrestrial ecosystem. (c) 2007 Elsevier Ltd. All rights reserved.
学科主题	Soil Science
收录类别	SCI
语种	英语
WOS记录号	WOS:000249538500010
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54617]
专题	沈阳应用生态研究所_沈阳应用生态研究所_期刊论文
推荐引用方式 GB/T 7714	Zhang, XD,He, HB,Amelung, W. A GC/MS method for the assessment of N-15 and C-13 incorporation into soil amino acid enantiomers[J]. SOIL BIOLOGY & BIOCHEMISTRY,2007,39(11):2785-2796.
APA	Zhang, XD,He, HB,&Amelung, W.(2007).A GC/MS method for the assessment of N-15 and C-13 incorporation into soil amino acid enantiomers.SOIL BIOLOGY & BIOCHEMISTRY,39(11),2785-2796.
MLA	Zhang, XD,et al."A GC/MS method for the assessment of N-15 and C-13 incorporation into soil amino acid enantiomers".SOIL BIOLOGY & BIOCHEMISTRY 39.11(2007):2785-2796.