Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils | |
Li, XY ; Zhang, HW ; Wu, M ; Su, ZC ; Zhang, CG | |
刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA |
2008 | |
卷号 | 20期号:9页码:1126-1131 |
关键词 | ammonia-oxidizing bacteria (AOB) PCR-DGGE acetochlor |
ISSN号 | 1001-0742 |
通讯作者 | Zhang, HW, Chinese Acad Sci, Inst Appl Ecol, Microbiolog Resource & Ecol Grp, Shenyang 110016, Peoples R China |
中文摘要 | Acetochlor is an increasingly used herbicide on corn in North China. Currently, the effect of acetochlor on soil ammonia-oxidizing bacteria (AOB) communities is not well documented. Here, we studied the diversity and community composition of AOB in soil amended with three concentrations of acetochlor (50, 150, 250 mg/kg) and the control (0 mg acetochlor/kg soil) in a microcosm experiment by PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and the phylogenetic analysis of excised DGGE bands. DGGE profiles showed that acetochlor had a stimulating effect on AOB at the early stage after acetochlor amended, and the order of intensity and duration is medium-acetochlor amended samples (AM) > low-acetochlor amended samples (AL) > high-acetochlor amended samples (AH). At the end of 60 d microcosm, acetochlor had a negative effect on the diversity of AOB. Cluster analysis of DGGE profiles showed that acetochlor had a greater effect on the community structure of AOB on day 60 than on day 1. The phylogenetic analysis revealed that all the sequences of excised DGGE bands were closely related to members of the genus Nitrosospira and formed two separate subclusters designated as subcluster 1 and subcluster 2 affiliated respectively with Clusters 3 and 4 in Nitrosospira as defined by Stephen. Some dominant AOB had a change from subcluster 2 to subcluster I with the incubation. The results showed that acetochlor had an effect on the AOB on a long-term basis and the chronic effect of acetochlor should be paid more attention in future. |
学科主题 | Environmental Sciences |
收录类别 | SCI |
资助信息 | Natural Science Foundation for Young Scientists of China [40701088] |
语种 | 英语 |
WOS记录号 | WOS:000259244600016 |
公开日期 | 2011-09-23 |
内容类型 | 期刊论文 |
源URL | [http://210.72.129.5/handle/321005/54387] |
专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
推荐引用方式 GB/T 7714 | Li, XY,Zhang, HW,Wu, M,et al. Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2008,20(9):1126-1131. |
APA | Li, XY,Zhang, HW,Wu, M,Su, ZC,&Zhang, CG.(2008).Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,20(9),1126-1131. |
MLA | Li, XY,et al."Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 20.9(2008):1126-1131. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论