High efficiency and simple preparation of polyacrylamide coated silica stationary phase for hydrophilic interaction liquid chromatography | |
Guo Y(郭勇)![]() ![]() ![]() ![]() | |
刊名 | Journal of Chromatography A
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2019 | |
卷号 | 1605期号:1页码:360357 |
英文摘要 | A polyacrylamide (PAM)-coated silica packing as a novel stationary phase for hydrophilic interaction liquid chromatography (HILIC) was produced firstly by thermal immobilization. The coated layer was formed by a simple and facile physical method that dipping silica spheres into a PAM solution and then stirring the mixture until the solution was evaporated absolutely, leaving a certain amount of PAM on the silica particles. Although there are no chemical bonds involved, the bleeding measurement and the background noise are acceptable. Polyacrylamide coated silica (PAM-Sil, mean diameter: 5 m) demonstrated a good hydrophilic property and excellent separation efficiency, which was observed especially for several high polar analytes such as saccharides. It is much higher than bare silica or amino columns under the same conditions. Moreover the obtained stationary phase demonstrated good stability under our operation conditions. The specific preparing method offers an easy and economical way to manipulate the thickness of PAM coating and can be extended as a universal way to produce various polymer coated stationary phases with dipping-heating/stirring-evaporating procedure. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.licp.cn/handle/362003/25747] ![]() |
专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
通讯作者 | Wang S(王帅); Wang YH(王玉环) |
作者单位 | 1.Standardization Research Institute of Petroleum Industry, Research Institute of Petroleum Exploration & Development 2.University of Chinese Academy of Sciences 3.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical |
推荐引用方式 GB/T 7714 | Guo Y,Liang XJ,Wang S,et al. High efficiency and simple preparation of polyacrylamide coated silica stationary phase for hydrophilic interaction liquid chromatography[J]. Journal of Chromatography A,2019,1605(1):360357. |
APA | Guo Y.,Liang XJ.,Wang S.,Wang L.,Bu H.,...&Wang LC.(2019).High efficiency and simple preparation of polyacrylamide coated silica stationary phase for hydrophilic interaction liquid chromatography.Journal of Chromatography A,1605(1),360357. |
MLA | Guo Y,et al."High efficiency and simple preparation of polyacrylamide coated silica stationary phase for hydrophilic interaction liquid chromatography".Journal of Chromatography A 1605.1(2019):360357. |
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