Effect of Cys, GSH, and pH on Mercury Release from Tibetan Medicine Zuotai, beta-HgS, and alpha-HgS in Artificial Gastrointestinal Juices

	Effect of Cys, GSH, and pH on Mercury Release from Tibetan Medicine Zuotai, beta-HgS, and alpha-HgS in Artificial Gastrointestinal Juices
	Zheng, Zhiyuan; Zhang, Ming; Xiao, Yuancan; Wei, Lixin; Li, Cen
刊名	BIOLOGICAL TRACE ELEMENT RESEARCH
	2018
卷号	184 期号:2
关键词	Tibetan medicine Zuotai Mercury release Mercuric sulfide L-Cysteine Glutathione pH
英文摘要	Zuotai, also named as "gTso thal", a known Tibetan medicinal mixture containing insoluble cubic crystal mercuric sulfide (beta-HgS), has been used to treat diseases with long history. The mercury release ratio from Zuotai in gastrointestinal environment is one determinant factor for its bioavailability and biological effect. However, the information is still scarce now. Therefore, the study was designed to investigate the effect of sulfhydryl biomolecules [l-cysteine (Cys) and glutathione (GSH)] and pH on mercury dissociation from Zuotai, beta-HgS, and hexagonal crystal mercuric sulfide (alpha-HgS) in artificial gastrointestinal juices or pure water with a 1:100 solid-liquid ratio. And, the digestion and peristalsis of gastrointestinal tract were simulated in vitro. The results showed the following trend for the mercury release ratio of Zuotai, artificial gastric juice > artificial intestinal juice > pure water, whereas the trend for beta-HgS and alpha-HgS was as follows, artificial intestinal fluid > artificial gastric fluid > pure water. The mercury release ratios of Zuotai, beta-HgS, and alpha-HgS significantly increased in artificial intestinal juice containing l-Cys or GSH compared to those without sulfhydryl biomolecules in the juice. However, in contrast to the results observed for beta-HgS and alpha-HgS, the mercury release ratio of Zuotai was reduced remarkably in pure water and artificial gastric juice with Cys or GSH. And, we found that strong acidic or strong alkaline environments promoted the dissociation of mercury from Zuotai, beta-HgS, and alpha-HgS. Taken together, current findings may contribute to other studies regarding clinical safety and bioavailability of the traditional drug Zuotai containing beta-HgS.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/59993]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zheng, Zhiyuan,Zhang, Ming,Xiao, Yuancan,et al. Effect of Cys, GSH, and pH on Mercury Release from Tibetan Medicine Zuotai, beta-HgS, and alpha-HgS in Artificial Gastrointestinal Juices[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2018,184(2).
APA	Zheng, Zhiyuan,Zhang, Ming,Xiao, Yuancan,Wei, Lixin,&Li, Cen.(2018).Effect of Cys, GSH, and pH on Mercury Release from Tibetan Medicine Zuotai, beta-HgS, and alpha-HgS in Artificial Gastrointestinal Juices.BIOLOGICAL TRACE ELEMENT RESEARCH,184(2).
MLA	Zheng, Zhiyuan,et al."Effect of Cys, GSH, and pH on Mercury Release from Tibetan Medicine Zuotai, beta-HgS, and alpha-HgS in Artificial Gastrointestinal Juices".BIOLOGICAL TRACE ELEMENT RESEARCH 184.2(2018).