beta-carboline alkaloids attenuate bleomycin induced pulmonary fibrosis in mice through inhibiting NF-kb/p65 phosphorylation and epithelial-mesenchymal transition

	beta-carboline alkaloids attenuate bleomycin induced pulmonary fibrosis in mice through inhibiting NF-kb/p65 phosphorylation and epithelial-mesenchymal transition
	Cui, Yulei; Jiang, Lei; Yu, Ruitao; Shao, Yun; Mei, Lijuan; Tao, Yanduo
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2019
卷号	243
关键词	beta-carboline alkaloids Pulmonary fibrosis NF-kb/p65 EMT Arenaria kansuensis
英文摘要	Ethnopharmacological relevance: The plant Arenaria kansuensis is used in traditional medicine to treat lung inflammation for a long time. However, the anti-pulmonary fibrosis effect and its corresponding bioactive constituents of this plant have not been studied extensively. Aim of the study: The purpose of this study was to investigate the anti-pulmonary fibrosis effect and its corresponding bioactive constituents of A. kansuensis and its possible mechanism. Materials and methods: In vivo experiment, the anti-pulmonary fibrosis effects of the fraction (Part1) enriched from ethyl acetate extracts of the whole plant A. kansuensis were evaluated through bleomycin (BLM)-induced pulmonary fibrosis mice (five groups, n = 10) daily at doses of 50, 100 and 150 mg/kg for 15 days. In vitro experiment, the anti-inflammation and reversed epithelial-mesenchymal transition (EMT) effect of 12 beta-carboline alkaloids isolated from Part1 were evaluated through lipopolysaccharide (LPS)-induced RAW264.7 inflammatory cell model and TGF-beta 1 induced A549 cell model. Results: In this study, a fraction named Part1 extracted from Arenaria kansuensis presented strong anti-pulmonary fibrosis effect at the dose of 150 mg/kg. Vivo experiments showed that the survival rate and body weight of mice significantly increased after Partl treatment. Part1 could significantly inhibit the initial of inflammation, deposition of collagen and expression of TGF-beta 1 and alpha-SMA, moreover, the expression of E-cadherin was significantly elevated after administration of Part1. All the cure effects of Part1 were in dose dependent manner. A total of 12 beta-carboline alkaloids were identified in Part1 and they all showed suppressive effect on inflammatory cytokines including MCP-1, TNF-alpha, IL-6 and IL-1 beta through inhibition of NF-kb/p65 phosphorylation, and that epithelial-mesenchymal transition (EMT) process was reversed by different compounds in different levels. The expression of indicators of EMT including alpha-SMA, vimentin and E-cadherin was significantly improved after given different beta-carboline alkaloids. Conclusions: This study showed that antifibrogenic effect of beta-carboline alkaloids was due to inhibiting the initial of inflammation through NF-kb/p65 pathway and reversing the process of EMT.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/59683]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Cui, Yulei,Jiang, Lei,Yu, Ruitao,et al. beta-carboline alkaloids attenuate bleomycin induced pulmonary fibrosis in mice through inhibiting NF-kb/p65 phosphorylation and epithelial-mesenchymal transition[J]. JOURNAL OF ETHNOPHARMACOLOGY,2019,243.
APA	Cui, Yulei,Jiang, Lei,Yu, Ruitao,Shao, Yun,Mei, Lijuan,&Tao, Yanduo.(2019).beta-carboline alkaloids attenuate bleomycin induced pulmonary fibrosis in mice through inhibiting NF-kb/p65 phosphorylation and epithelial-mesenchymal transition.JOURNAL OF ETHNOPHARMACOLOGY,243.
MLA	Cui, Yulei,et al."beta-carboline alkaloids attenuate bleomycin induced pulmonary fibrosis in mice through inhibiting NF-kb/p65 phosphorylation and epithelial-mesenchymal transition".JOURNAL OF ETHNOPHARMACOLOGY 243(2019).