Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization

doi:10.1016/j.bpj.2019.09.012

	Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization
	Long M(龙勉)2,3,4 ; Lv SQ(吕守芹)2,3,4 ; Hu WH(胡文慧)1,3,4; Chen SB(陈深宝)2,3,4; Hu JR(胡锦荣)2,3,4
刊名	BIOPHYSICAL JOURNAL
	2019-10-15
卷号	117 期号:8 页码:1405-1418
ISSN号	0006-3495
DOI	10.1016/j.bpj.2019.09.012
通讯作者	Lu, Shouqin(lsq@imech.ac.cn) ; Long, Mian(mlong@imech.ac.cn)
英文摘要	Global cytoskeleton reorganization is well-recognized when cells are exposed to distinct mechanical stimuli, but the localized responses at a specified region of a cell are still unclear. In this work, we mapped the cell-surface mechanical property of single cells in situ before and after static point loading these cells using atomic force microscopy in PeakForce-Quantitative Nano Mechanics mode. Cell-surface stiffness was elevated at a maximum of 1.35-fold at the vicinity of loading site, indicating an enhanced structural protection of the cortex to the cell. Mechanical modeling also elucidated the structural protection from the stiffened cell cortex, in which 9-15% and 10-19% decrease of maximum stress and strain of the nucleus were obtained. Furthermore, the flat-ended atomic force microscopy probes were used to capture cytoskeleton reorganization after point loading quantitatively, revealing that the larger the applied force and the longer the loading time are, the more pronounced cytoskeleton reorganization is. Also, point loading using a microneedle combined with real-time confocal microscopy uncovered the fast dynamics of actin cytoskeleton reorganization for actin-stained live cells after point loading (<10 s). These results furthered the understandings in the transmission of localized mechanical forces into an adherent cell.
分类号	一类/力学重要期刊
资助项目	National Key Research and Development Program of China[2016YFA0501601] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31570942] ; Frontier Science Key Project of Chinese Science Academy[QYZDJ-SSW-JSC018] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB22040101]
WOS关键词	ATOMIC-FORCE MICROSCOPY ; INDUCED CYTOSKELETAL REORGANIZATION ; CELL STIFFNESS ; CYTOCHALASIN-D ; ENDOTHELIAL-CELLS ; PLASMA-MEMBRANE ; CANCER-CELLS ; STRESS ; DECONDENSATION ; ORGANIZATION
WOS研究方向	Biophysics
语种	英语
WOS记录号	WOS:000490144100004
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; Frontier Science Key Project of Chinese Science Academy ; Strategic Priority Research Program of Chinese Academy of Sciences
其他责任者	Lu, Shouqin ; Long, Mian
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/80670]
专题	力学研究所_国家微重力实验室
作者单位	1.Guizhou Med Univ, Key Lab Biol & Med Engn, Immune Cells & Antibody Engn Res Ctr Guizhou Prov, Guiyang, Guizhou, Peoples R China 2.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China; 3.Chinese Acad Sci, CAS Ctr Excellence Complex Syst Mech, Inst Mech, Beijing, Peoples R China; 4.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab,Beijing Key, Beijing, Peoples R China;
推荐引用方式 GB/T 7714	Long M,Lv SQ,Hu WH,et al. Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization[J]. BIOPHYSICAL JOURNAL,2019,117(8):1405-1418.
APA	龙勉,吕守芹,胡文慧,陈深宝,&胡锦荣.(2019).Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization.BIOPHYSICAL JOURNAL,117(8),1405-1418.
MLA	龙勉,et al."Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization".BIOPHYSICAL JOURNAL 117.8(2019):1405-1418.