Adsorption of Phospholipids at the Air-Water Surface

doi:10.1016/j.bpj.2019.08.022

CORC > 力学研究所 > 中国科学院力学研究所 > 非线性力学国家重点实验室

	Adsorption of Phospholipids at the Air-Water Surface
	Hu GQ(胡国庆)5 ; Zuo YY 1,2; Tang JY 2; Xu L2 ; Bai X(白轩)3,4,5
刊名	BIOPHYSICAL JOURNAL
	2019-10-01
卷号	117 期号:7 页码:1224-1233
ISSN号	0006-3495
DOI	10.1016/j.bpj.2019.08.022
通讯作者	Zuo, Yi Y.(yzuo@hawaii.edu) ; Hu, Guoqing(ghu@zju.edu.cn)
英文摘要	Phospholipids are ubiquitous components of biomembranes and common biomaterials used in many bioengineering applications. Understanding adsorption of phospholipids at the air-water surface plays an important role in the study of pulmonary surfactants and cell membranes. To date, however, the biophysical mechanisms of phospholipid adsorption are still unknown. It is challenging to reveal the molecular structure of adsorbed phospholipid films. Using combined experiments with constrained drop surfactometry and molecular dynamics simulations, here, we studied the biophysical mechanisms of dipalmitoylphosphatidylcholine (DPPC) adsorption at the air-water surface. It was found that the DPPC film adsorbed from vesicles showed distinct equilibrium surface tensions from the DPPC monolayer spread via organic solvents. Our simulations revealed that only the outer leaflet of the DPPC vesicle is capable of unzipping and spreading at the air-water surface, whereas the inner leaflet remains intact and forms an inverted micelle to the interfacial monolayer. This inverted micelle increases the local curvature of the monolayer, thus leading to a loosely packed monolayer at the air-water surface and hence a higher equilibrium surface tension. These findings provide novel insights, to our knowledge, into the mechanism of the phospholipid and pulmonary surfactant adsorption and may help understand the structure-function correlation in biomembranes.
分类号	一类/力学重要期刊
资助项目	Natural Science Foundation of China[11832017] ; Natural Science Foundation of China[11572334] ; Natural Science Foundation of China[91543125] ; Chinese Academy of Sciences Key Research Program of Frontier Sciences[QYZDB-SSW-JSC036] ; Chinese Academy of Sciences Strategic Priority Research Program[XDB22040403] ; National Science Foundation[CBET-1604119] ; Hawai'i Community Foundation[18ADVC-90802]
WOS关键词	COARSE-GRAINED MODEL ; PULMONARY SURFACTANT ; LUNG SURFACTANT ; LIPID-BILAYERS ; COMPETITIVE ADSORPTION ; HYDROPHOBIC SURFACES ; TENSION MEASUREMENTS ; MOLECULAR-MECHANISM ; DYNAMIC ADSORPTION ; SP-B
WOS研究方向	Biophysics
语种	英语
WOS记录号	WOS:000488457600006
资助机构	Natural Science Foundation of China ; Chinese Academy of Sciences Key Research Program of Frontier Sciences ; Chinese Academy of Sciences Strategic Priority Research Program ; National Science Foundation ; Hawai'i Community Foundation
其他责任者	Zuo, Yi Y. ; Hu, Guoqing
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/80661]
专题	力学研究所_非线性力学国家重点实验室
作者单位	1.Univ Hawaii Manoa, Dept Pediat, John A Burns Sch Med, Honolulu, HI 96822 USA 2.Univ Hawaii Manoa, Dept Mech Engn, John A Burns Sch Med, Honolulu, HI 96822 USA; 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China; 4.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing, Peoples R China; 5.Zhejiang Univ, Dept Engn Mech, Hangzhou, Zhejiang, Peoples R China;
推荐引用方式 GB/T 7714	Hu GQ,Zuo YY,Tang JY,et al. Adsorption of Phospholipids at the Air-Water Surface[J]. BIOPHYSICAL JOURNAL,2019,117(7):1224-1233.
APA	胡国庆,Zuo YY,Tang JY,Xu L,&白轩.(2019).Adsorption of Phospholipids at the Air-Water Surface.BIOPHYSICAL JOURNAL,117(7),1224-1233.
MLA	胡国庆,et al."Adsorption of Phospholipids at the Air-Water Surface".BIOPHYSICAL JOURNAL 117.7(2019):1224-1233.