Responses of thioredoxin 1 and thioredoxin-related protein 14 mRNAs to cadmium and copper stresses in Venerupis philippinarum

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 污染过程与控制实验室

	Responses of thioredoxin 1 and thioredoxin-related protein 14 mRNAs to cadmium and copper stresses in Venerupis philippinarum
	Wang, Qing1 ; Ning, Xuanxuan 2; Chen, Leilei 3; Pei, Dong 4; Zhao, Jianmin1 ; Zhang, Linbao 1,5; Liu, Xiaoli 1,5; Wu, Huifeng1
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
	2011-09-01
卷号	154 期号:3 页码:154-160
关键词	Cadmium Copper Thioredoxin 1 Thioredoxin-related protein 14 Venerupis philippinarum
通讯作者	Zhao, JM, Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, CAS Shandong Prov Key Lab Coastal Zone Environm P, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn ; hfwu@yic.ac.cn
产权排序	[Wang, Q; Zhao, JM; Zhang, LB; Liu, XL; Wu, HF] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, CAS Shandong Prov Key Lab Coastal Zone Environm P, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Ning, XX] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [Chen, LL] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; [Pei, D] China Agr Univ, Yantai 264670, Peoples R China; [Zhang, LB; Liu, XL] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
中文摘要	Thioredoxin (abbreviated as Trx) is an important ubiquitous disulfide reductase, which can protect organisms against various oxidative stresses. In the present study, thioredoxin 1 (named as VpTrx1) and thioredoxin-related protein (named as VpTrp14) were identified from Venerupis philippinarum, respectively. Similar to most Trx1s, VpTrx1 possessed all conserved features critical for the fundamental structure and function of Trx1s, such as the conserved catalytic residues (C-G-P-C), but lacked the other cysteine residues, while VpTrp14 contained the conserved motif (C-P-D-C). Quantitative Real-time PCR assay showed that VpTrx1 and VpTrp14 transcripts were distributed in a wide array of tissues most abundantly expressed in the hepatopancreas. The expression of VpTrp14 mRNA in the hepatopancreas was significantly up-regulated after exposure to 10 and 40 mu g/L Cd, while the VpTrx1 expression level was kept relatively constant. Both the expression levels of VpTrx1 and VpTrp14 in the hepatopancreas were induced after exposure to Cu, and increased to the peak value at 96 h under the 40 mu g/L Cu exposure. These results showed that VpTrp14 transcripts responded to metal stress more acutely than VpTrx1, and both Trxs responded to Cu stress more sensitively than Cd. Together, it was suggested that VpTrx1 and VpTrp14 perhaps played important roles in the antioxidant responses against metal stress in V. philippinarum. (C) 2011 Elsevier Inc. All rights reserved.
英文摘要	Thioredoxin (abbreviated as Trx) is an important ubiquitous disulfide reductase, which can protect organisms against various oxidative stresses. In the present study, thioredoxin 1 (named as VpTrx1) and thioredoxin-related protein (named as VpTrp14) were identified from Venerupis philippinarum, respectively. Similar to most Trx1s, VpTrx1 possessed all conserved features critical for the fundamental structure and function of Trx1s, such as the conserved catalytic residues (C-G-P-C), but lacked the other cysteine residues, while VpTrp14 contained the conserved motif (C-P-D-C). Quantitative Real-time PCR assay showed that VpTrx1 and VpTrp14 transcripts were distributed in a wide array of tissues most abundantly expressed in the hepatopancreas. The expression of VpTrp14 mRNA in the hepatopancreas was significantly up-regulated after exposure to 10 and 40 mu g/L Cd, while the VpTrx1 expression level was kept relatively constant. Both the expression levels of VpTrx1 and VpTrp14 in the hepatopancreas were induced after exposure to Cu, and increased to the peak value at 96 h under the 40 mu g/L Cu exposure. These results showed that VpTrp14 transcripts responded to metal stress more acutely than VpTrx1, and both Trxs responded to Cu stress more sensitively than Cd. Together, it was suggested that VpTrx1 and VpTrp14 perhaps played important roles in the antioxidant responses against metal stress in V. philippinarum. (C) 2011 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Endocrinology & Metabolism; Toxicology; Zoology
研究领域[WOS]	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
关键词[WOS]	MARINE-INVERTEBRATES ; OXIDATIVE STRESS ; GENE-EXPRESSION ; DISK ABALONE ; BOHAI SEA ; ANTIOXIDANT ; IDENTIFICATION ; SUPERFAMILY ; APOPTOSIS ; DNA
收录类别	SCI
资助信息	NSFC[30901115]; Chinese Academy of Sciences; SDSFC[ZR2009CZ008]; Key Laboratory for Ecological Environment in Coastal Areas[201011]; Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology[201115]; State Oceanic Administration
语种	英语
WOS记录号	WOS:000293994200003
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5124]
专题	烟台海岸带研究所_污染过程与控制实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, CAS Shandong Prov Key Lab Coastal Zone Environm P, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China 3.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 4.China Agr Univ, Yantai 264670, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qing,Ning, Xuanxuan,Chen, Leilei,et al. Responses of thioredoxin 1 and thioredoxin-related protein 14 mRNAs to cadmium and copper stresses in Venerupis philippinarum[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2011,154(3):154-160.
APA	Wang, Qing.,Ning, Xuanxuan.,Chen, Leilei.,Pei, Dong.,Zhao, Jianmin.,...&Wu, Huifeng.(2011).Responses of thioredoxin 1 and thioredoxin-related protein 14 mRNAs to cadmium and copper stresses in Venerupis philippinarum.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,154(3),154-160.
MLA	Wang, Qing,et al."Responses of thioredoxin 1 and thioredoxin-related protein 14 mRNAs to cadmium and copper stresses in Venerupis philippinarum".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 154.3(2011):154-160.