Elucidating the toxicity targets of beta-ionone on photosynthetic system of Microcystis aeruginosa NIES-843 (Cyanobacteria)

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 藻类生物学及应用研究中心 > 期刊论文

	Elucidating the toxicity targets of beta-ionone on photosynthetic system of Microcystis aeruginosa NIES-843 (Cyanobacteria)
	Shao, Jihai 1,2; Xu, Yao 3; Wang, Zhongjie 1,4; Jiang, Yongguang 1,4; Yu, Gongliang 1; Peng, Xin 1; Li, Renhui 1
刊名	AQUATIC TOXICOLOGY
	2011-07-01
卷号	104 期号:1-2 页码:48-55
关键词	Microcystis aeruginosa Photosynthesis Gene expression Chlorophyll a fluorescence
ISSN号	0166-445X
通讯作者	Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; shaojihai@yahoo.com.cn ; reli@ihb.ac.cn
中文摘要	In order to explore the potential targets of toxicity of beta-ionone on the photosynthetic system of Microcystis aeruginosa, the polyphasic rise in chlorophyll a (Chl a) fluorescence transient and transcript expression for key genes in photosystem II (PSII) of M. aeruginosa NIES-843 were studied. The EC(50) value of beta-ionone on M. aeruginosa NIES-843 was found to be 21.23 +/- 1.87 mg/L It was shown that beta-Ionone stress can lead to a decrease in pigment content of M. aeruginosa NIES-843 cells, and that carotenoids were more sensitive to beta-ionone stress than Chl a. The normalized Chl a fluorescence transients were slightly decreased at 6.67 and 10 mg/L beta-ionone, but significantly increased at 15, 22.5 and 33.75 mg/L There was no significant variation on transcript expression of psbA and psbO at a concentration of 6.67 mg/L beta-ionone. but they were down-regulated at 22.5 mg/L Ultrastructural examination by transmission electron microscopy indicated that the thylakoids were distorted, and the thylakoid membrane stacks began to collapse when M. aeruginosa NIES-843 was exposed to beta-ionone at a concentration of 22.5 and 33.75 mg/L Our results indicate that the reaction centre of PS II and the electron transport at the acceptor side of PS II are the targets responsible for the toxicity of beta-ionone on the PS II of M. aeruginosa NIES-843. (C) 2011 Elsevier B.V. All rights reserved.
英文摘要	In order to explore the potential targets of toxicity of beta-ionone on the photosynthetic system of Microcystis aeruginosa, the polyphasic rise in chlorophyll a (Chl a) fluorescence transient and transcript expression for key genes in photosystem II (PSII) of M. aeruginosa NIES-843 were studied. The EC(50) value of beta-ionone on M. aeruginosa NIES-843 was found to be 21.23 +/- 1.87 mg/L It was shown that beta-Ionone stress can lead to a decrease in pigment content of M. aeruginosa NIES-843 cells, and that carotenoids were more sensitive to beta-ionone stress than Chl a. The normalized Chl a fluorescence transients were slightly decreased at 6.67 and 10 mg/L beta-ionone, but significantly increased at 15, 22.5 and 33.75 mg/L There was no significant variation on transcript expression of psbA and psbO at a concentration of 6.67 mg/L beta-ionone. but they were down-regulated at 22.5 mg/L Ultrastructural examination by transmission electron microscopy indicated that the thylakoids were distorted, and the thylakoid membrane stacks began to collapse when M. aeruginosa NIES-843 was exposed to beta-ionone at a concentration of 22.5 and 33.75 mg/L Our results indicate that the reaction centre of PS II and the electron transport at the acceptor side of PS II are the targets responsible for the toxicity of beta-ionone on the PS II of M. aeruginosa NIES-843. (C) 2011 Elsevier B.V. All rights reserved.
学科主题	Marine & Freshwater Biology; Toxicology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Marine & Freshwater Biology ; Toxicology
研究领域[WOS]	Marine & Freshwater Biology ; Toxicology
关键词[WOS]	CHLOROPHYLL-A FLUORESCENCE ; MYRIOPHYLLUM-SPICATUM ; SPIRULINA-PLATENSIS ; ALLELOPATHIC ACTIVITY ; PSII PHOTOCHEMISTRY ; DROUGHT STRESS ; PHOTOSYSTEM-II ; PLANTS ; GROWTH ; INHIBITION
收录类别	SCI
资助信息	NSFC-JST[30821140542]; Hunan Provincial Natural Science Foundation of China[10JJ6045]; National Water Science and Technology[2009ZX07101-013-02]
语种	英语
WOS记录号	WOS:000292949700006
公开日期	2011-11-09
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16377]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China 3.Nanjing Normal Univ, Coll Geog Sci, Dept Environm Sci & Engn, Nanjing 210046, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Shao, Jihai,Xu, Yao,Wang, Zhongjie,et al. Elucidating the toxicity targets of beta-ionone on photosynthetic system of Microcystis aeruginosa NIES-843 (Cyanobacteria)[J]. AQUATIC TOXICOLOGY,2011,104(1-2):48-55.
APA	Shao, Jihai.,Xu, Yao.,Wang, Zhongjie.,Jiang, Yongguang.,Yu, Gongliang.,...&Li, Renhui.(2011).Elucidating the toxicity targets of beta-ionone on photosynthetic system of Microcystis aeruginosa NIES-843 (Cyanobacteria).AQUATIC TOXICOLOGY,104(1-2),48-55.
MLA	Shao, Jihai,et al."Elucidating the toxicity targets of beta-ionone on photosynthetic system of Microcystis aeruginosa NIES-843 (Cyanobacteria)".AQUATIC TOXICOLOGY 104.1-2(2011):48-55.