The effects of x-ray radiation on the eye development of zebrafish

doi:10.1177/0960327114522278

	The effects of x-ray radiation on the eye development of zebrafish
	Zhou R(周蓉)
刊名	Human and Experimental Toxicology
	2014-10-15
卷号	33 期号:10 页码:1040-1050
关键词	X-Ray eye development zebrafish cell apoptosis reactive oxygen species microophthalmia
ISSN号	0960-3271
DOI	10.1177/0960327114522278
英文摘要	The toxic effects of x-ray radiation on eye development was measured using zebrafish as a model organism. Zebrafish embryos at 8 h post-fertilization (hpf) were irradiated using X-rays at doses of 1, 2, 4, and 8 Gy. At 24 and 48 hpf, x-ray radiation induced a significant increase in reactive oxygen species (ROS) content and cell apoptotic signals. Both of these increases were dose dependent and there were significant positive relationships between them at 24 hpf. At 48 and 72 hpf, the increase of ROS concentration can be eliminated by increasing activities of superoxide dismutase and catalase. Although the ROS generated by x-ray radiation caused a significant increase in cell apoptosis at 24 and 48 hpf, the cellular layers of the retina and lens formation in the irradiated groups were not significantly disrupted at 144 hpf compared with the control group, with the exception of a heterogeneous distribution of the cells in inner nuclear cell layer and a significant decrease in the diameters of whole eyes after 8 Gy irradiation. X-Ray radiation at later stages of gastrulation may not cause distinct optic complications; however, there is still a risk of microophthalmia at high doses of irradiation.
资助项目	Scientific Technology Research Projects of Gansu Province[0702NKDA045] ; Scientific Technology Research Projects of Gansu Province[0806RJYA020]
WOS关键词	STRAND BREAK REPAIR ; TUMOR STEM-CELLS ; OXIDATIVE STRESS ; IONIZING-RADIATION ; VERTEBRATE EMBRYO ; DANIO-RERIO ; DNA-DAMAGE ; EXPOSURE ; MODEL ; IRRADIATION
WOS研究方向	Toxicology
语种	英语
出版者	SAGE PUBLICATIONS LTD
WOS记录号	WOS:000343382400005
公开日期	2014-12-17
内容类型	期刊论文
源URL	[http://ir.impcas.ac.cn/handle/113462/12574]
专题	近代物理研究所_辐射医学研究室
推荐引用方式 GB/T 7714	Zhou R. The effects of x-ray radiation on the eye development of zebrafish[J]. Human and Experimental Toxicology,2014,33(10):1040-1050.
APA	Zhou R.(2014).The effects of x-ray radiation on the eye development of zebrafish.Human and Experimental Toxicology,33(10),1040-1050.
MLA	Zhou R."The effects of x-ray radiation on the eye development of zebrafish".Human and Experimental Toxicology 33.10(2014):1040-1050.