Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia

doi:10.1038/s41598-017-02049-9

	Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia
	Shi, Wengui 2,4; Wang, Jufang2 ; Ding, Nan2 ; Xian, Cory J.3; Ma, Huiping 1; Chen, Keming 1; Wei, Wenjun 2,4; Gao, Yuhai 1; Zhou, Jian 1; He, Jinpeng2
刊名	SCIENTIFIC REPORTS
	2017-05-12
卷号	7 页码:12
ISSN号	2045-2322
DOI	10.1038/s41598-017-02049-9
英文摘要	It is well documented that microgravity in space environment leads to bone loss in astronauts. These physiological changes have also been validated by human and animal studies and modeled in cell-based analogs. However, the underlying mechanisms are elusive. In the current study, we identified a novel phenomenon that primary cilia (key sensors and functioning organelles) of rat calvarial osteoblasts (ROBs) gradually shrank and disappeared almost completely after exposure to simulated microgravity generated by a random positioning machine (RPM). Along with the abrogation of primary cilia, the differentiation, maturation and mineralization of ROBs were inhibited. We also found that the disappearance of primary cilia was prevented by treating ROBs with cytochalasin D, but not with LiCl or dynein light chain Tctex-type 1 (Dynlt1) siRNA. The repression of the differentiation, maturation and mineralization of ROBs was effectively offset by cytochalasin D treatment in microgravity conditions. Blocking ciliogenesis using intraflagellar transport protein 88 (IFT88) siRNA knockdown inhibited the ability of cytochalasin D to counteract this reduction of osteogenesis. These results indicate that the abrogation of primary cilia may be responsible for the microgravity's inhibition on osteogenesis. Reconstruction of primary cilia may become a potential strategy against bone loss induced by microgravity.
资助项目	International Science & Technology Cooperation Program of China[2015DFR30940] ; National Natural Sciences Foundation of China[81270963] ; National Natural Sciences Foundation of China[81471090] ; NHMRC[1042105]
WOS关键词	MESENCHYMAL STEM-CELLS ; RANDOM POSITIONING MACHINE ; OSTEOGENIC DIFFERENTIATION ; BONE-FORMATION ; FLUID SHEAR ; BED REST ; LENGTH ; CILIOGENESIS ; EXPRESSION ; MATURATION
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000401262400059
资助机构	International Science & Technology Cooperation Program of China ; National Natural Sciences Foundation of China ; NHMRC
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/44782]
专题	近代物理研究所_生物物理研究室
通讯作者	Wang, Jufang; Chen, Keming
作者单位	1.Lanzhou Command CPLA, Lanzhou Gen Hosp, Inst Orthopaed, Lanzhou 730050, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Gansu Key Lab Space Radiobiol, Lanzhou 730000, Peoples R China 3.Univ South Australia, Sch Pharm & Med Sci, Sansom Inst Hlth Res, Adelaide, SA 5001, Australia 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Shi, Wengui,Wang, Jufang,Ding, Nan,et al. Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia[J]. SCIENTIFIC REPORTS,2017,7:12.
APA	Shi, Wengui.,Wang, Jufang.,Ding, Nan.,Xian, Cory J..,Ma, Huiping.,...&Xie, Yanfang.(2017).Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia.SCIENTIFIC REPORTS,7,12.
MLA	Shi, Wengui,et al."Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia".SCIENTIFIC REPORTS 7(2017):12.