Mutagenic effects of carbon ion beam irradiations on dry Lotus japonicus seeds

doi:10.1016/j.nimb.2016.06.021

	Mutagenic effects of carbon ion beam irradiations on dry Lotus japonicus seeds
	Luo, Shanwei 2,3; Zhou, Libin2 ; Li, Wenjian2 ; Du, Yan2 ; Yu, Lixia2,3 ; Feng, Hui 2,3; Mu, Jinhu 2,3; Chen, Yuze 1
刊名	NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
	2016-09-15
卷号	383 页码:123-128
关键词	Carbon ion beam Lotus japonicus Mutation breeding ISSR
ISSN号	0168-583X
DOI	10.1016/j.nimb.2016.06.021
英文摘要	Carbon ion beam irradiation is a powerful method for creating mutants and has been used in crop breeding more and more. To investigate the effects of carbon ion beams on Lotus japonicus, dry seeds were irradiated by 80 MeV/u carbon ion beam at dosages of 0, 100, 200, 300, 400, 500 and 600 Gy. The germination rate, survival rate and root length of M-1 populations were explored and the dose of 400 Gy was selected as the median lethal dose (LD50) for a large-scale mutant screening. Among 2472 M-2 plants, 127 morphological mutants including leaf, stem, flower and fruit phenotypic variation were found, and the mutation frequency was approximately 5.14%. Inter simple sequence repeat (ISSR) assays were utilized to investigate the DNA polymorphism between seven mutants and eight plants without phenotypic variation from M-2 populations. No remarkable differences were detected between these two groups, and the total polymorphic rate was 0.567%. (C) 2016 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[11205218] ; National Natural Science Foundation of China[11275171] ; National Natural Science Foundation of China[11405234] ; Strategic Priority Research Program of the Chinese Academy of Sciences (CAS)[XDA08040111] ; Knowledge Innovation Project of CAS[KJCX2-EW-N05] ; CAS "Light of West China" Program[29Y506020] ; Youth Innovation Promotion Association of CAS[2015337] ; CAS "Young Scholars of West China" Program[Y606020]
WOS关键词	REPEAT ISSR POLYMORPHISM ; ARABIDOPSIS-THALIANA ; GENETIC DIVERSITY ; IONIZING-RADIATION ; MUTATION-RATE ; DNA MARKERS ; HEAVY-IONS ; MUTANTS ; PLANTS ; GERMINATION
WOS研究方向	Instruments & Instrumentation ; Nuclear Science & Technology ; Physics
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000383007300016
资助机构	National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) ; Knowledge Innovation Project of CAS ; CAS "Light of West China" Program ; Youth Innovation Promotion Association of CAS ; CAS "Young Scholars of West China" Program
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/43193]
专题	近代物理研究所_生物物理研究室
通讯作者	Zhou, Libin
作者单位	1.Gansu Agr Univ, Coll Life Sci & Technol, 1 Yingmen Village, Lanzhou 730070, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Biophys Grp, 509 Nanchang Rd, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Luo, Shanwei,Zhou, Libin,Li, Wenjian,et al. Mutagenic effects of carbon ion beam irradiations on dry Lotus japonicus seeds[J]. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS,2016,383:123-128.
APA	Luo, Shanwei.,Zhou, Libin.,Li, Wenjian.,Du, Yan.,Yu, Lixia.,...&Chen, Yuze.(2016).Mutagenic effects of carbon ion beam irradiations on dry Lotus japonicus seeds.NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS,383,123-128.
MLA	Luo, Shanwei,et al."Mutagenic effects of carbon ion beam irradiations on dry Lotus japonicus seeds".NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS 383(2016):123-128.