Photosynthetic Effect in Selenastrum capricornutum Progeny after Carbon-Ion Irradiation

doi:10.1371/journal.pone.0149381

	Photosynthetic Effect in Selenastrum capricornutum Progeny after Carbon-Ion Irradiation
	Ma, Liang1 ; Du, Yan1 ; Lu, Dong1 ; Li, Xin1 ; Wang, Jie 1,2; Wang, Jufang1 ; Hu, Guangrong 3; Fan, Yong 3; Li, Fuli 3; Chen, Jihong1
刊名	PLOS ONE
	2016-02-26
卷号	11 页码:14
ISSN号	1932-6203
DOI	10.1371/journal.pone.0149381
英文摘要	A large proportion of mutants with altered pigment features have been obtained via exposure to heavy-ion beams, a technique that is efficient for trait improvement in the breeding of plants and algae. However, little is known about the underlying mechanisms by which the photosynthetic pigments are altered by heavy-ion irradiation. In our study, the photosynthetic characteristics of progenies from carbon-ion irradiated Selenastrum capricornutum were investigated. Five progenies deficient in chlorophyll a were isolated after carbon-ion exposure. Photosynthetic characteristics, photoprotection capacity and gene expression of the light-harvesting complex in these progenies were further characterized by the measurement of chlorophyll fluorescence parameters (Fv/Fm,.PSII, NPQ, ETR), the de-epoxidation state of the xanthophyll cycle, the amount of lutein and quantitative real-time PCR. High maximum quantum yield of photosystem II at day 10 and high thermal dissipation ability were observed in progenies #23 and #37 under normal culture condition. Progenies #18, #19 and #20 showed stronger resistance against high levels of light steps than the control group (612-1077 mu mol photons m(-2) s(-1), p< 0.05). The progenies #20 and #23 exhibited strong photoprotection by thermal dissipation and quenching of (3)Chl* after 24 h of high light treatment. The mRNA levels of Lhcb5, Lhcbm5 and Lhcbm1 of the light-harvesting complex revealed markedly differential expression in the five progenies irradiated by carbon-ion beams. This work indicates that photosynthetic efficiency, photoprotection ability and the expression of light-harvesting antennae in unicellular green algae can be markedly influenced by irradiation. To our knowledge, this is the first report on changes in the photosynthetic pigments of green algae after treatment with carbon-ion beams.
资助项目	National Nature Science Foundation of China[U1232126] ; Chinese Academy of Sciences, China[Y306010XB0] ; Science and Technology Service Network Initiative Project of the Chinese Academy of Sciences[Y506050STS]
WOS关键词	CHLOROPHYLL FLUORESCENCE ; CHLAMYDOMONAS-REINHARDTII ; XANTHOPHYLL CYCLE ; HIGHER-PLANTS ; MUTATION-RATE ; IN-VIVO ; LIGHT ; PHOTOPROTECTION ; ARABIDOPSIS ; MUTANTS
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000371274400019
资助机构	National Nature Science Foundation of China ; Chinese Academy of Sciences, China ; Science and Technology Service Network Initiative Project of the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/41947]
专题	近代物理研究所_生物物理研究室
通讯作者	Wang, Jufang
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Gansu Key Lab Space Radiobiol & Microbial Resourc, Lanzhou, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Ma, Liang,Du, Yan,Lu, Dong,et al. Photosynthetic Effect in Selenastrum capricornutum Progeny after Carbon-Ion Irradiation[J]. PLOS ONE,2016,11:14.
APA	Ma, Liang.,Du, Yan.,Lu, Dong.,Li, Xin.,Wang, Jie.,...&Li, Wenjian.(2016).Photosynthetic Effect in Selenastrum capricornutum Progeny after Carbon-Ion Irradiation.PLOS ONE,11,14.
MLA	Ma, Liang,et al."Photosynthetic Effect in Selenastrum capricornutum Progeny after Carbon-Ion Irradiation".PLOS ONE 11(2016):14.