Ring chromosome aberrations in human lymphocytes induced by 5-20 Gy carbon ion irradiation

	Ring chromosome aberrations in human lymphocytes induced by 5-20 Gy carbon ion irradiation
	Wang Biqian 1,2; Wang Zhuanzi1 ; Li Wenjian1 ; Xing Junzhen 3
刊名	NUCLEAR SCIENCE AND TECHNIQUES
	2010-12-01
卷号	21 页码:362-365
关键词	Carbon ion beams Human lymphocytes Prematurely condensed ring chromosome
ISSN号	1001-8042
英文摘要	In this paper, we study the biological response induced by heavy ions irradiation of high dose, human peripheral blood lymphocytes are irradiated in vitro by the carbon ions of LET=35 keV/mu m, and the chromosome aberrations at absorbed doses of 0-20 Gy are analyzed by the calyculin A-induced premature chromosome condensation (PCC). The frequencies of PCC-rings at the stage of G2/M-phase increase steeply with radiation doses up to 20 Gy at a rate of 0.017 Gy(-1). The G2-PCC index remains more than 5% up to 15 Gy, and 3% after 20 Gy, this is high enough to score a substantial number of chromosome spreads without dose-rate effect. The results show that the calyculin A-induced PCC technique is suitable for analyzing the chromosome damage induced by carbon ions irradiation of high dose.
资助项目	National Natural Science Foundation of China[10875153] ; Chinese Academy of Sciences for Postgraduate students[O906350YZ0] ; Chinese Academy of Sciences[O806260XB0] ; Chinese Academy of Sciences[O806270XBB]
WOS关键词	PREMATURELY CONDENSED CHROMOSOMES ; SIMPLE BIODOSIMETRY METHOD ; DOSE RADIATION-EXPOSURE ; INDUCTION ; GAMMA ; PCC ; CELLS
WOS研究方向	Nuclear Science & Technology ; Physics
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000287642700009
资助机构	National Natural Science Foundation of China ; Chinese Academy of Sciences for Postgraduate students ; Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/33254]
专题	近代物理研究所_生物物理研究室
通讯作者	Wang Zhuanzi
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Xiangning Hosp Shanxi Prov, Xiangning 042100, Peoples R China
推荐引用方式 GB/T 7714	Wang Biqian,Wang Zhuanzi,Li Wenjian,et al. Ring chromosome aberrations in human lymphocytes induced by 5-20 Gy carbon ion irradiation[J]. NUCLEAR SCIENCE AND TECHNIQUES,2010,21:362-365.
APA	Wang Biqian,Wang Zhuanzi,Li Wenjian,&Xing Junzhen.(2010).Ring chromosome aberrations in human lymphocytes induced by 5-20 Gy carbon ion irradiation.NUCLEAR SCIENCE AND TECHNIQUES,21,362-365.
MLA	Wang Biqian,et al."Ring chromosome aberrations in human lymphocytes induced by 5-20 Gy carbon ion irradiation".NUCLEAR SCIENCE AND TECHNIQUES 21(2010):362-365.