A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase

doi:10.1007/s41365-016-0157-8

	A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase
	Liu, Jing1 ; Li, Wen-Jian1 ; Chen, Ji-Hong1 ; Xiao, Guo-Qing1 ; Wang, Shu-Yang1,3 ; Jiang, Bo-Ling 1,4; Wang, Yu-Chen 1,2; Dong, Miao-Ying 1; Xu, Fu-Qiang 1; Hu, Wei 1
刊名	NUCLEAR SCIENCE AND TECHNIQUES
	2017
卷号	28 页码:7
关键词	High-throughput screening method Aspergillus niger H11 Cellulase C-12(6+) ion beam
ISSN号	1001-8042
DOI	10.1007/s41365-016-0157-8
英文摘要	In this study, a high-throughput screening method was established through the 24-square deep-well microliter plate (MTP) fermentation and micro-plate detection for large-scale screening of the mutants. It was suitable for screening a large number of mutants and improving the breeding efficiency after heavy-ion beam irradiation. Seventeen strains showed higher cellulase activity compared with the initial strain after the screening of plate and MTP fermentation. The filter paper activity and beta-glucosidase activity of Aspergillus niger H11201 had increased 38.74 and 63.23 % separately compared with A. niger H11 by shaking flask fermentation, and it was genetically stable after being passaged to nine generations. The results indicate that the high-throughput screening method can be used for the quick breeding of A. niger with high cellulase activity.
资助项目	National Natural Science Foundation of China[11305225] ; Science and Technology Service Network Initiative[KFJ-EW-STS-086]
WOS关键词	IRRADIATION ; REPRESSION ; INDUCTION ; ASSAY
WOS研究方向	Nuclear Science & Technology ; Physics
语种	英语
出版者	SPRINGER SINGAPORE PTE LTD
WOS记录号	WOS:000393150100004
资助机构	National Natural Science Foundation of China ; Science and Technology Service Network Initiative
内容类型	期刊论文
源URL	[http://ir.impcas.ac.cn/handle/113462/23528]
专题	近代物理研究所_生物物理研究室近代物理研究所_兰州重离子研究装置
通讯作者	Wang, Shu-Yang
作者单位	1.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Peoples R China 2.Gansu Agr Univ, Lanzhou 730000, Gansu, Peoples R China 3.Lanzhou Univ, 222 South Tianshui Rd, Lanzhou 730000, Gansu, Peoples R China 4.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jing,Li, Wen-Jian,Chen, Ji-Hong,et al. A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase[J]. NUCLEAR SCIENCE AND TECHNIQUES,2017,28:7.
APA	Liu, Jing.,Li, Wen-Jian.,Chen, Ji-Hong.,Xiao, Guo-Qing.,Wang, Shu-Yang.,...&Hu, Wei.(2017).A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase.NUCLEAR SCIENCE AND TECHNIQUES,28,7.
MLA	Liu, Jing,et al."A high-throughput screening method for breeding Aspergillus niger with C-12(6+) ion beam-improved cellulase".NUCLEAR SCIENCE AND TECHNIQUES 28(2017):7.