No significant level of inheritable interchromosomal aberrations in the progeny of bystander primary human fibroblasts after alpha particle irradiation

doi:10.1016/j.asr.2012.09.020

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	No significant level of inheritable interchromosomal aberrations in the progeny of bystander primary human fibroblasts after alpha particle irradiation
	Hei, Tom K.1; Zhu, Jiayun2,3 ; Hu, Burong3 ; Zhou, Hongning 1
刊名	ADVANCES IN SPACE RESEARCH
	2013-02-01
卷号	51 页码:450-457
关键词	Genomic Instability Bystander Effect Chromatid Break Mfish Alpha-particle Irradiation
ISSN号	0273-1177
DOI	10.1016/j.asr.2012.09.020
文献子类	Article
英文摘要	A major concern for bystander effects is the probability that normal healthy cells adjacent to the irradiated cells become genomically unstable and undergo further carcinogenesis after therapeutic irradiation or space mission where astronauts are exposed to low dose of heavy ions. Genomic instability is a hallmark of cancer cells. In the present study, two irradiation protocols were performed in order to ensure pure populations of bystander cells and the genomic instability in their progeny were investigated. After irradiation, chromosomal aberrations of cells were analyzed at designated time points using G(2) phase premature chromosome condensation (G(2)-PCC) coupled with Giemsa staining and with multiplex fluorescent in situ hybridization (mFISH). Our Giemsa staining assay demonstrated that elevated yields of chromatid breaks were induced in the progeny of pure bystander primary fibroblasts up to 20 days after irradiation. mFISH assay showed no significant level of inheritable interchromosomal aberrations were induced in the progeny of the bystander cell groups, while the fractions of gross aberrations (chromatid breaks or chromosomal breaks) significantly increased in some bystander cell groups. These results suggest that genomic instability occurred in the progeny of the irradiation associated bystander normal fibroblasts exclude the inheritable interchromosomal aberration. (C) 2012 COSPAR. Published by Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[31170803]
WOS关键词	SISTER-CHROMATID EXCHANGES ; HIGH-LET RADIATION ; NORMAL HUMAN-CELLS ; GENOMIC INSTABILITY ; MAMMALIAN-CELLS ; DNA-DAMAGE ; CHROMOSOMAL INSTABILITY ; IONIZING-RADIATION ; OXIDATIVE STRESS ; EXPOSURE
WOS研究方向	Astronomy & Astrophysics ; Geology ; Meteorology & Atmospheric Sciences
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000314386000012
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/48347]
专题	近代物理研究所_空间辐射生物研究室
通讯作者	Hu, Burong
作者单位	1.Columbia Univ, Coll Phys & Surg, Ctr Radiol Res, New York, NY 10032 USA 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Hei, Tom K.,Zhu, Jiayun,Hu, Burong,et al. No significant level of inheritable interchromosomal aberrations in the progeny of bystander primary human fibroblasts after alpha particle irradiation[J]. ADVANCES IN SPACE RESEARCH,2013,51:450-457.
APA	Hei, Tom K.,Zhu, Jiayun,Hu, Burong,&Zhou, Hongning.(2013).No significant level of inheritable interchromosomal aberrations in the progeny of bystander primary human fibroblasts after alpha particle irradiation.ADVANCES IN SPACE RESEARCH,51,450-457.
MLA	Hei, Tom K.,et al."No significant level of inheritable interchromosomal aberrations in the progeny of bystander primary human fibroblasts after alpha particle irradiation".ADVANCES IN SPACE RESEARCH 51(2013):450-457.