Reprogramming mediated radio-resistance of 3D-grown cancer cells

doi:10.1093/jrr/rrv018

CORC > 近代物理研究所 > 中国科学院近代物理研究所 > 空间辐射生物研究室

	Reprogramming mediated radio-resistance of 3D-grown cancer cells
	Hu, Burong2 ; Xue, Gang 2,3; Ren, Zhenxin2 ; Grabham, Peter W.1; Chen, Yaxiong2 ; Zhu, Jiayun2 ; Du, Yarong2 ; Pan, Dong 2,3; Li, Xiaoman 2,3
刊名	JOURNAL OF RADIATION RESEARCH
	2015-07-01
卷号	56 页码:656-662
关键词	3D growth microenvironment matrigel reprogramming beta-catenin radio-resistance
ISSN号	0449-3060
DOI	10.1093/jrr/rrv018
英文摘要	In vitro 3D growth of tumors is a new cell culture model that more closely mimics the features of the in vivo environment and is being used increasingly in the field of biological and medical research. It has been demonstrated that cancer cells cultured in 3D matrices are more radio-resistant compared with cells in monolayers. However, the mechanisms causing this difference remain unclear. Here we show that cancer cells cultured in a 3D microenvironment demonstrated an increase in cells with stem cell properties. This was confirmed by the finding that cells in 3D cultures upregulated the gene and protein expression of the stem cell reprogramming factors such as OCT4, SOX2, NANOG, LIN28 and miR-302a, compared with cells in monolayers. Moreover, the expression of beta-catenin, a regulating molecule of reprogramming factors, also increased in 3D-grown cancer cells. These findings suggest that cancer cells were reprogrammed to become stem cell-like cancer cells in a 3D growth culture microenvironment. Since cancer stem cell-like cells demonstrate an increased radio-resistance and chemo-resistance, our results offer a new perspective as to why. Our findings shed new light on understanding the features of the 3D growth cell model and its application in basic research into clinical radiotherapy and medicine.
资助项目	Hundred Talent Program of the Chinese Academy of Sciences[Y163100BR0] ; 973 program from the Department of Chinese Science[2010CB834201] ; National Natural Science Foundation of China[31170803] ; Major Project Specialized for Infectious Diseases of the Chinese Health and Family Planning Commission[2014ZX10004002] ; National Key Scientific Instrument and Equipment Development Project of China[2012YQ03014210]
WOS关键词	EMBRYONIC STEM-CELLS ; MAMMARY EPITHELIAL-CELLS ; LOW ATTACHMENT SURFACES ; TUMOR MICROENVIRONMENT ; EXTRACELLULAR-MATRIX ; GENE-EXPRESSION ; TISSUE ARCHITECTURE ; CULTURE MODELS ; FORCED GROWTH ; 3D SPHERES
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000360990700007
资助机构	Hundred Talent Program of the Chinese Academy of Sciences ; 973 program from the Department of Chinese Science ; National Natural Science Foundation of China ; Major Project Specialized for Infectious Diseases of the Chinese Health and Family Planning Commission ; National Key Scientific Instrument and Equipment Development Project of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/40623]
专题	近代物理研究所_空间辐射生物研究室
通讯作者	Hu, Burong
作者单位	1.Columbia Univ, Coll Phys & Surg, Ctr Radiol Res, New York, NY 10032 USA 2.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Dept Space Radiobiol, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Burong,Xue, Gang,Ren, Zhenxin,et al. Reprogramming mediated radio-resistance of 3D-grown cancer cells[J]. JOURNAL OF RADIATION RESEARCH,2015,56:656-662.
APA	Hu, Burong.,Xue, Gang.,Ren, Zhenxin.,Grabham, Peter W..,Chen, Yaxiong.,...&Li, Xiaoman.(2015).Reprogramming mediated radio-resistance of 3D-grown cancer cells.JOURNAL OF RADIATION RESEARCH,56,656-662.
MLA	Hu, Burong,et al."Reprogramming mediated radio-resistance of 3D-grown cancer cells".JOURNAL OF RADIATION RESEARCH 56(2015):656-662.