Modulation of microRNAs by ionizing radiation in human gastric cancer

doi:10.3892/or.2014.3246

CORC > 近代物理研究所 > 中国科学院近代物理研究所 > 空间辐射生物研究室

	Modulation of microRNAs by ionizing radiation in human gastric cancer
	He JP(何进鹏)
刊名	ONCOLOGY REPORTS
	2014-05-13
卷号	32 期号:5 页码:1-7
关键词	microRNA ionizing radiation gastric cancer miR-300 miR-642
ISSN号	1021-335X
DOI	10.3892/or.2014.3246
英文摘要	Gastric cancer is one of the most common cancers in China. Although surgery is the primary therapeutic method, radiotherapy has become an integral part, particularly in the early and intermediate stages of gastric cancer. microRNAs (miRNAs) are involved in the regulation of diverse cellular processes in response to intrinsic and extrinsic stress. A change in miRNA expression profile has been identified in various types of tumor cells in response to radiation; however, there is no relevant information concerning gastric cancer. In the present study, we investigated the miRNA profiles of two clinical gastric cancer samples exposed to X-rays using miRNA microarray. We found that 16 miRNAs were downregulated and 2 miRNAs were upregulated significantly in both irradiated samples when compared with the unirradiated samples. Decreases in the levels of miR-300 and miR-642 expression were confirmed by qRT-PCR in more clinical samples and in cultured cell lines. We predicted the targets of the two miRNAs with Target Scan and classified all the candidate targets with Gene Ontology, which indicated that both miR-300 and miR-642 potentially regulate cellular radiation response by modulating apoptosis, cell cycle regulation and DNA damage and repair pathway-related genes. Cell cycle assay and immunofluorescence assay demonstrated that miR-300 regulates radiation-induced G2 cell cycle arrest and DNA damage repair. In conclusion, our findings indicate that ionizing radiation modulates the miRNA expression profile, and the changes in several specific miRNAs such as miR-300 have the potential to be used in the treatment, diagnosis and prognosis of gastric cancer.
资助项目	National Natural Science Foundation of China[31270895] ; National Natural Science Foundation of China[81272454] ; National Natural Science Foundation of China[U1232125]
WOS关键词	CELL-LINES ; MULTIDRUG-RESISTANCE ; PROSTATE-CANCER ; TARGETING PTEN ; DNA-REPAIR ; EXPRESSION ; APOPTOSIS ; PROFILES ; PROLIFERATION ; BIOGENESIS
WOS研究方向	Oncology
语种	英语
出版者	SPANDIDOS PUBL LTD
WOS记录号	WOS:000338908700043
公开日期	2014-12-17
内容类型	期刊论文
源URL	[http://ir.impcas.ac.cn/handle/113462/12570]
专题	近代物理研究所_空间辐射生物研究室
推荐引用方式 GB/T 7714	He JP. Modulation of microRNAs by ionizing radiation in human gastric cancer[J]. ONCOLOGY REPORTS,2014,32(5):1-7.
APA	He JP.(2014).Modulation of microRNAs by ionizing radiation in human gastric cancer.ONCOLOGY REPORTS,32(5),1-7.
MLA	He JP."Modulation of microRNAs by ionizing radiation in human gastric cancer".ONCOLOGY REPORTS 32.5(2014):1-7.