The Effects of C-12(6+) Irradiation on Cell Cycle, Apoptosis, and Expression of Caspase-3 in the Human Lung Cancer Cell Line H1299

doi:10.1089/cbr.2011.1037

	The Effects of C-12(6+) Irradiation on Cell Cycle, Apoptosis, and Expression of Caspase-3 in the Human Lung Cancer Cell Line H1299
	Wang, ZR 1; Gao, QX 1; Wang, ZR (reprint author), Lanzhou Univ, Sch Life Sci, 222 S Tianshui Rd, Lanzhou 730000, Peoples R China.; Gao, L 1; Che, TJ 1; Du, WJ 1; Li, Q1 ; Xu, H 1
刊名	CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
	2012
卷号	27 页码:113-118
关键词	Radiation Activation Exposure Pathways Death Ion
ISSN号	1084-9785
DOI	10.1089/cbr.2011.1037
英文摘要	Introduction: We aimed to investigate the effects of C-12(6+) irradiation on the cell cycle and apoptosis as well as the associated mechanisms in the human lung cancer cell line H1299. Methods: After irradiation with different doses of C-12(6+) for varying lengths of incubation, the changes in H1299 cells were assayed by flow cytometry and the microculture tetrazolium test. The expression of caspase-3 was detected by immunocytochemistry, western blot, and reverse transcription-polymerase chain reaction (RT-PCR). Results: The G(2)/M phase was blocked after treatment with 1 and 2 Gy at the 12-hour time point, and the most obvious block of G(2)/M occurred after treatment with 2 and 4 Gy at the 24-hour time point in a dose-dependent manner. The apoptosis rate increased with increasing radiation dose and reached a peak after the cells were irradiated with 2 Gy and incubated for 48 hours. In addition, the RT-PCR, western blot, and ICC results showed that irradiation with C-12(6+) significantly increased the expression of caspase-3 compared with the control group (p < 0.05). Conclusions: Irradiation of H1299 cells with C-12(6+) induced apoptosis and significantly inhibited their growth through heavy ion irradiation-mediated activation of the caspase-3 pathway. Our results show that caspase-3 may play an important role in radiation-induced apoptosis through a p53-independent pathway.
URL标识	查看原文
WOS关键词	RADIATION ; ACTIVATION ; EXPOSURE ; PATHWAYS ; DEATH ; ION
WOS研究方向	Oncology ; Research & Experimental Medicine ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	MARY ANN LIEBERT INC
WOS记录号	WOS:000301440900002
资助机构	National Basic Research Program of China (973 Program) [2010CB834203] ; National Basic Research Program of China (973 Program) [2010CB834203] ; Key Project of National Natural Science Foundation of China [10835011] ; Key Project of National Natural Science Foundation of China [10835011] ; National Natural Science Foundation of China [10905080, 11075191] ; National Natural Science Foundation of China [10905080, 11075191] ; Natural Science Foundation of Gansu Province of China [1010RJZA007] ; Natural Science Foundation of Gansu Province of China [1010RJZA007] ; Science and Technology Development Project of Lanzhou City [2008-sr-10, 2009-2-12] ; Science and Technology Development Project of Lanzhou City [2008-sr-10, 2009-2-12]
内容类型	期刊论文
源URL	[http://ir.impcas.ac.cn/handle/113462/18587]
专题	近代物理研究所_医学物理研究室近代物理研究所_生物物理研究室
通讯作者	Wang, ZR (reprint author), Lanzhou Univ, Sch Life Sci, 222 S Tianshui Rd, Lanzhou 730000, Peoples R China.
作者单位	1.[Li, Ping 2.Liu, Xinguo 3.Jin, Xiaodong 4.Dai, Zhongying 5.Li, Qiang] Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 6.[Matsumoto, Yoshitaka 7.Furusawa, Yoshiya] Natl Inst Radiol Sci, Heavy Ion Radiobiol Res Grp, Chiba 2638555, Japan 8.[Li, Ping 9.Dai, Zhongying] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 10.[Li, Ping
推荐引用方式 GB/T 7714	Wang, ZR,Gao, QX,Wang, ZR ,et al. The Effects of C-12(6+) Irradiation on Cell Cycle, Apoptosis, and Expression of Caspase-3 in the Human Lung Cancer Cell Line H1299[J]. CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,2012,27:113-118.
APA	Wang, ZR.,Gao, QX.,Wang, ZR .,Gao, L.,Che, TJ.,...&Xu, H.(2012).The Effects of C-12(6+) Irradiation on Cell Cycle, Apoptosis, and Expression of Caspase-3 in the Human Lung Cancer Cell Line H1299.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,27,113-118.
MLA	Wang, ZR,et al."The Effects of C-12(6+) Irradiation on Cell Cycle, Apoptosis, and Expression of Caspase-3 in the Human Lung Cancer Cell Line H1299".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS 27(2012):113-118.