Spatial Dynamics of DNA Damage Response Protein Foci along the Ion Trajectory of High-LET Particles

doi:10.1667/RR2592.1

	Spatial Dynamics of DNA Damage Response Protein Foci along the Ion Trajectory of High-LET Particles
	Kugler, Alexandra 4; Friedl, Anna A.4; Dollinger, Guenther 2; Friedland, Werner 3; Drexler, Guido A.4; Du, Guanghua1,5 ; Greubel, Christoph 2; Hable, Volker 2; Kruecken, Reiner 5; Tonelli, Laura 5
刊名	RADIATION RESEARCH
	2011-12-01
卷号	176 页码:706-715
ISSN号	0033-7587
DOI	10.1667/RR2592.1
英文摘要	Du, G., Drexler, G. A., Friedland, W., Greubel, C., Hable, V., Krucken, R., Kugler, A, Tonelli, L., Friedl, A. A. and Dollinger, G. Spatial Dynamics of DNA Damage Response Protein Foci along the Ion Trajectory of High-LET Particles. Radiat. Res. 176, 706-715 (2011). High-linear energy transfer (LET) ion irradiation of cell nuclei induces complex and severe DNA lesions, and foci of repair proteins are formed densely along the ion trajectory. To efficiently discriminate the densely distributed/overlapping foci along the ion trajectory, a focus recognition algorithm called FociPicker3D based on a local fraction thresholding technique was developed. We analyzed high-resolution 3D immunofluorescence microscopic focus images and obtained the kinetics and spatial development of gamma-H2AX, 53BP1 and phospho-NBS1 foci in BJ1-hTERT cells irradiated with 55 MeV carbon ions and compared the results with the dynamics of double-strand break (DSB) distributions simulated using the PARTRAC model. Clusters consisting of several foci were observed along the ion trajectory after irradiation. The spatial dynamics of the protein foci supports that the foci clusters are not formed by neighboring foci but instead originate from the DSB cluster damage induced by high-LET radiations. (C) 2011 by Radiation Research Society
资助项目	DFG Cluster of Excellence ; Munich-Centre for Advanced Photonics ; CAS[Y150310BR0] ; BfS[StSch 4450] ; Maier Leibnitz Laboratorium of the Ludwig-Maximilians-Universitat Munchen ; Technische Universitat Munchen
WOS关键词	DOUBLE-STRAND BREAKS ; HISTONE H2AX ; CHROMOSOME DOMAINS ; REPAIR PROTEINS ; MICROSCOPY ; GAMMA-H2AX ; RADIATION ; CELLS ; PHOSPHORYLATION ; RECRUITMENT
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Biophysics ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	RADIATION RESEARCH SOC
WOS记录号	WOS:000297904000002
资助机构	DFG Cluster of Excellence ; Munich-Centre for Advanced Photonics ; CAS ; BfS ; Maier Leibnitz Laboratorium of the Ludwig-Maximilians-Universitat Munchen ; Technische Universitat Munchen
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/33816]
专题	近代物理研究所_实验物理中心
通讯作者	Du, Guanghua
作者单位	1.CAS, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 2.Univ Bundeswehr Munchen, LRT 2, D-85579 Neubiberg, Germany 3.Helmholtz Zentrum Munchen, Inst Radiat Protect, D-85764 Neuherberg, Germany 4.Univ Munich, Dept Radiat Oncol, D-80336 Munich, Germany 5.Tech Univ Munich, Phys Dept E12, D-85748 Garching, Germany
推荐引用方式 GB/T 7714	Kugler, Alexandra,Friedl, Anna A.,Dollinger, Guenther,et al. Spatial Dynamics of DNA Damage Response Protein Foci along the Ion Trajectory of High-LET Particles[J]. RADIATION RESEARCH,2011,176:706-715.
APA	Kugler, Alexandra.,Friedl, Anna A..,Dollinger, Guenther.,Friedland, Werner.,Drexler, Guido A..,...&Tonelli, Laura.(2011).Spatial Dynamics of DNA Damage Response Protein Foci along the Ion Trajectory of High-LET Particles.RADIATION RESEARCH,176,706-715.
MLA	Kugler, Alexandra,et al."Spatial Dynamics of DNA Damage Response Protein Foci along the Ion Trajectory of High-LET Particles".RADIATION RESEARCH 176(2011):706-715.