Early effects of low dose C-12(6+) ion or X-ray irradiation on human peripheral blood lymphocytes

doi:10.1016/j.asr.2009.09.024

CORC > 近代物理研究所 > 中国科学院近代物理研究所 > 近代物理研究所知识存储（2010之前）

	Early effects of low dose C-12(6+) ion or X-ray irradiation on human peripheral blood lymphocytes
	Chen, Yingtai 3; Zhang, Yawei 7; Liu, Hongliang 5; Zhu, Zijiang 5; Zhang, Xiaowei 1; Ren, Jinyu 6; Chen, Xuezhong 5; Xie, Yi4 ; Zhang, Hong4 ; Li, Yumin 2
刊名	ADVANCES IN SPACE RESEARCH
	2010-04-01
卷号	45 期号:7 页码:832-838
关键词	C-12(6+) ion X-ray Human Lymphocyte Spare
ISSN号	0273-1177
DOI	10.1016/j.asr.2009.09.024
英文摘要	The aim of this study was to estimate the acute effects of low dose C-12(6+) ions or X-ray radiation on human immune function. The human peripheral blood lymphocytes (HPBL) of seven healthy donors were exposed to 0.05 Gy C-12(6+) ions or X-ray radiation and cell responses were measured at 24 h after exposure. The cytotoxic activities of HPBL were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT); the percentages of T and NK cells subsets were detected by flow cytometry; mRNA expression of interleukin (IL)-2, tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma were examined by real time quantitative RT-PCR (qRT-PCR); and these cytokines protein levels in supematant of cultured cells were assayed by enzyme-linked immunosorbent assays (ELISA). The results showed that the cytotoxic activity of HPBL, mRNA expression of IL-2, IFN-gamma and TNF-alpha in HPBL and their protein levels in supernatant were significantly increased at 24 h after exposure to 0.05 Gy C-12(6+) ions radiation and the effects were stronger than observed for X-ray exposure. However, there was no significant change in the percentage of T and NK cells subsets of HPBL. These results suggested that 0.05 Gy high linear energy transfer (LET) C-12(6+) radiation was a more effective approach to host immune enhancement than that of low LET X-ray. We conclude that cytokines production might be used as sensitive indicators of acute response to LDL (C) 2009 COSPAR. Published by Elsevier Ltd. All rights reserved.
资助项目	National Science Foundation of China[30770639] ; National Science Foundation of China[30870364] ; National Institute of Health (NIH)[ID43TW008323-01] ; National Institute of Health (NIH)[ID43TW007864-01]
WOS关键词	TOTAL-BODY IRRADIATION ; EXTRATHYMIC T-CELLS ; IONIZING-RADIATION ; ADAPTIVE RESPONSE ; INTERFERON-GAMMA ; MICE ; INTERLEUKIN-2 ; GROWTH ; MELANOMA ; HORMESIS
WOS研究方向	Astronomy & Astrophysics ; Geology ; Meteorology & Atmospheric Sciences
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000276179300002
资助机构	National Science Foundation of China ; National Institute of Health (NIH)
公开日期	2011-04-20
内容类型	期刊论文
源URL	[http://ir.imp.cas.cn/handle/113462/8001]
专题	近代物理研究所_近代物理研究所知识存储（2010之前）
通讯作者	Li, Yumin
作者单位	1.Lanzhou Univ, Cent Lab, Hosp 1, Lanzhou 730000, Gansu, Peoples R China 2.Lanzhou Univ, Dept Gen Surg, Hosp 2, Lanzhou 730030, Gansu, Peoples R China 3.Lanzhou Univ, Coll Clin Med, Lanzhou 730000, Gansu, Peoples R China 4.Chinese Acad Sci, Dept Radiol, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 5.Inst Med Sci Gansu Prov, Lanzhou 730050, Gansu, Peoples R China 6.Univ Kansas, Kidney Inst, Med Ctr, Kansas City, KS 66160 USA 7.Yale Univ, Sch Publ Hlth, New Haven, CT 06520 USA
推荐引用方式 GB/T 7714	Chen, Yingtai,Zhang, Yawei,Liu, Hongliang,et al. Early effects of low dose C-12(6+) ion or X-ray irradiation on human peripheral blood lymphocytes[J]. ADVANCES IN SPACE RESEARCH,2010,45(7):832-838.
APA	Chen, Yingtai.,Zhang, Yawei.,Liu, Hongliang.,Zhu, Zijiang.,Zhang, Xiaowei.,...&Li, Yumin.(2010).Early effects of low dose C-12(6+) ion or X-ray irradiation on human peripheral blood lymphocytes.ADVANCES IN SPACE RESEARCH,45(7),832-838.
MLA	Chen, Yingtai,et al."Early effects of low dose C-12(6+) ion or X-ray irradiation on human peripheral blood lymphocytes".ADVANCES IN SPACE RESEARCH 45.7(2010):832-838.