High-efficiency transfer and expression of AdCMV-p53 in human hepatocellular carcinoma cells induced by low-dose carbon-ion radiation

doi:10.1097/MEG.0b013e3282f945e8

CORC > 近代物理研究所 > 中国科学院近代物理研究所 > 近代物理研究所知识存储（2010之前）

	High-efficiency transfer and expression of AdCMV-p53 in human hepatocellular carcinoma cells induced by low-dose carbon-ion radiation
	Duan, Xin 1,3; Liu, Bing 1,2,3; Zhang, Hong1 ; Zhou, Qingming 1,3; Luo, Xiaohong 2; Xie, Yi1,3 ; Hao, Jifang 1
刊名	EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY
	2008-09-01
卷号	20 期号:9 页码:860-864
关键词	adenovirus vector carbon-ion beam gene therapy irradiation p53
ISSN号	0954-691X
DOI	10.1097/MEG.0b013e3282f945e8
英文摘要	Objective To investigate whether the irradiation with C-beam could enhance adenovirus-mediated transfer and expression of p53 in human hepatocellular carcinoma. Materials and methods HepG2 cells were exposed to C-beam or gamma-ray and then infected with replicationdeficient adenovirus recombinant vectors containing human wild-type p53 or green fluorescent protein, respectively. The transfer efficiency and expression level of the exogenous gene were detected by flow cytometric analysis. Cell survival fraction was detected by clonogenic assay. Results The transfer frequency in C-beam or gamma-irradiated groups increased by 50-83% and 5.7-38.0% compared with the control, respectively (P < 0.05). Compared with C-beam alone, p53 alone, and gamma-ray with p53, the percentages of p53 positive cells for 1 Gy C-beam with p53 increased by 56.0-72.0%, 63.5-82.0%, and 31.3-72.5% on first and third day after the treatments, respectively (P < 0.05). The survival fractions for the 2Gy C-bearn and AdCMV-p53 infection groups decreased to similar to 2%. Conclusion C-beam irradiation could significantly promote AdCMV-green fluorescent protein transfer and expression of p53.
资助项目	National Natural Science Foundation of China[10675151] ; Key Scientific Technology Research Project of Gansu Province[2GS052-A43-008-02] ; Nature Science Foundation of Gansu Province[3ZS061-A25-021]
WOS关键词	WILD-TYPE P53 ; HUMAN-GENE-THERAPY ; IONIZING-RADIATION ; MEMBRANE-PERMEABILITY ; MAMMALIAN-CELLS ; GLIOMA-CELLS ; ADENOVIRUS ; RADIOTHERAPY ; REPORTER ; CANCER
WOS研究方向	Gastroenterology & Hepatology
语种	英语
出版者	LIPPINCOTT WILLIAMS & WILKINS
WOS记录号	WOS:000259344200008
资助机构	National Natural Science Foundation of China ; Key Scientific Technology Research Project of Gansu Province ; Nature Science Foundation of Gansu Province
公开日期	2010-10-29
内容类型	期刊论文
源URL	[http://ir.imp.cas.cn/handle/113462/6323]
专题	近代物理研究所_近代物理研究所知识存储（2010之前）
通讯作者	Zhang, Hong
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Dept Med Phys, Lanzhou 730000, Peoples R China 2.Lanzhou Command Ctr Dis Prevent & Control, Lanzhou, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Duan, Xin,Liu, Bing,Zhang, Hong,et al. High-efficiency transfer and expression of AdCMV-p53 in human hepatocellular carcinoma cells induced by low-dose carbon-ion radiation[J]. EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY,2008,20(9):860-864.
APA	Duan, Xin.,Liu, Bing.,Zhang, Hong.,Zhou, Qingming.,Luo, Xiaohong.,...&Hao, Jifang.(2008).High-efficiency transfer and expression of AdCMV-p53 in human hepatocellular carcinoma cells induced by low-dose carbon-ion radiation.EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY,20(9),860-864.
MLA	Duan, Xin,et al."High-efficiency transfer and expression of AdCMV-p53 in human hepatocellular carcinoma cells induced by low-dose carbon-ion radiation".EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY 20.9(2008):860-864.