Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation

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	Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation
	Li Ning1,2,3 ; Zhang Hong1,2 ; Wang Yanling1,2,3 ; Wang Xiaohu 4; Hao Jifang 1,2
刊名	NUCLEAR SCIENCE AND TECHNIQUES
	2009-04-01
卷号	20 期号:2 页码:87-92
关键词	DNA-dependent protein kinase (DNA-PK) Cell cycle checkpoint Apoptosis Radiosensitivity
ISSN号	1001-8042
英文摘要	In this paper, we study the ability of DNA-PK-deficient (M059J) and -proficient (M059K) cells to undergo the rate of cellular proliferation, cell cycle distribution and apoptosis after 10 Gy X-ray irradiation, and the role of DNA-PK in radiosensitivity. The results showed that M059J cells exhibited hyper-radiosensitivity compared with M059K cells. A strong G2 phase arrest was observed in M059J cells post irradiation. Significant accumulation in the G2 phase in M059J cells was accompanied by apoptosis at 12 h. Altogether, the data suggested that DNA-PK may have two roles in mammalian cells after DNA damage, a role in DNA DSB repair and a second role in DNA-damaged cells to traverse a G2 checkpoint, by which DNA-PK may affect cellular sensitivity to ionizing radiation.
资助项目	National Natural Science Foundation of China[10675151] ; Chinese Academy of Sciences[0860260XBO]
WOS关键词	DOUBLE-STRAND BREAKS ; CATALYTIC SUBUNIT ; MAMMALIAN-CELLS ; P53-DEPENDENT APOPTOSIS ; M059K CELLS ; REPAIR ; CYCLE ; PATHWAYS ; DEATH ; MICE
WOS研究方向	Nuclear Science & Technology ; Physics
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000266620000005
资助机构	National Natural Science Foundation of China ; Chinese Academy of Sciences
公开日期	2010-10-29
内容类型	期刊论文
源URL	[http://ir.imp.cas.cn/handle/113462/6309]
专题	近代物理研究所_近代物理研究所知识存储（2010之前）
通讯作者	Zhang Hong
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Gansu Tumor Hosp, Dept Radiotherapy, Lanzhou 730050, Peoples R China
推荐引用方式 GB/T 7714	Li Ning,Zhang Hong,Wang Yanling,et al. Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation[J]. NUCLEAR SCIENCE AND TECHNIQUES,2009,20(2):87-92.
APA	Li Ning,Zhang Hong,Wang Yanling,Wang Xiaohu,&Hao Jifang.(2009).Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation.NUCLEAR SCIENCE AND TECHNIQUES,20(2),87-92.
MLA	Li Ning,et al."Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation".NUCLEAR SCIENCE AND TECHNIQUES 20.2(2009):87-92.