An efficient in vitro method for mass propagation of Potentilla potaninii wolf
He, W. T.; Hou, S. W.; Wang, C. Y.
刊名IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
2006-09-01
卷号42期号:5页码:415-417
关键词callus induction micropropagation organogenesis plant regeneration Potentilla potaninii Wolf
ISSN号1054-5476
DOI10.1079/IVP2006800
英文摘要This study reports a method for high-frequency shoot organogenesis and plant establishment of Potentilla potaninii Wolf. Hypocotyl and cotyledon explants of P. potaninii were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of benzyladenine (BA) and alpha-naphthaleneacetic acid (NAA) to induce adventitious shoot formation for micropropagation. The highest frequency of adventitious shoot regeneration was achieved from hypocotyl and cotyledon explants grown on MS medium supplemented with 5.0 mg l(-1) BA and 1.0 mg l(-1) NAA. The regenerated shoots rooted most efficiently on half-strength MS medium supplemented with 1.0 mg l(-1) NAA and 0.5 mg l(-1) indole-3-acetic acid or indole-3-butyric acid. The acclimatized plants with normal morphology and growth characters flowered and set seeds in the following year.
WOS研究方向Plant Sciences ; Cell Biology ; Developmental Biology
语种英语
出版者CABI PUBLISHING
WOS记录号WOS:000242217900009
公开日期2010-10-29
内容类型期刊论文
源URL[http://ir.imp.cas.cn/handle/113462/6111]  
专题近代物理研究所_近代物理研究所知识存储(2010之前)
通讯作者Hou, S. W.
作者单位1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714
He, W. T.,Hou, S. W.,Wang, C. Y.. An efficient in vitro method for mass propagation of Potentilla potaninii wolf[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2006,42(5):415-417.
APA He, W. T.,Hou, S. W.,&Wang, C. Y..(2006).An efficient in vitro method for mass propagation of Potentilla potaninii wolf.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,42(5),415-417.
MLA He, W. T.,et al."An efficient in vitro method for mass propagation of Potentilla potaninii wolf".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 42.5(2006):415-417.
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