An efficient in vitro method for mass propagation of Potentilla potaninii wolf | |
He, W. T.; Hou, S. W.; Wang, C. Y. | |
刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
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2006-09-01 | |
卷号 | 42期号:5页码:415-417 |
关键词 | callus induction micropropagation organogenesis plant regeneration Potentilla potaninii Wolf |
ISSN号 | 1054-5476 |
DOI | 10.1079/IVP2006800 |
英文摘要 | This study reports a method for high-frequency shoot organogenesis and plant establishment of Potentilla potaninii Wolf. Hypocotyl and cotyledon explants of P. potaninii were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of benzyladenine (BA) and alpha-naphthaleneacetic acid (NAA) to induce adventitious shoot formation for micropropagation. The highest frequency of adventitious shoot regeneration was achieved from hypocotyl and cotyledon explants grown on MS medium supplemented with 5.0 mg l(-1) BA and 1.0 mg l(-1) NAA. The regenerated shoots rooted most efficiently on half-strength MS medium supplemented with 1.0 mg l(-1) NAA and 0.5 mg l(-1) indole-3-acetic acid or indole-3-butyric acid. The acclimatized plants with normal morphology and growth characters flowered and set seeds in the following year. |
WOS研究方向 | Plant Sciences ; Cell Biology ; Developmental Biology |
语种 | 英语 |
出版者 | CABI PUBLISHING |
WOS记录号 | WOS:000242217900009 |
公开日期 | 2010-10-29 |
内容类型 | 期刊论文 |
源URL | [http://ir.imp.cas.cn/handle/113462/6111] ![]() |
专题 | 近代物理研究所_近代物理研究所知识存储(2010之前) |
通讯作者 | Hou, S. W. |
作者单位 | 1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China |
推荐引用方式 GB/T 7714 | He, W. T.,Hou, S. W.,Wang, C. Y.. An efficient in vitro method for mass propagation of Potentilla potaninii wolf[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2006,42(5):415-417. |
APA | He, W. T.,Hou, S. W.,&Wang, C. Y..(2006).An efficient in vitro method for mass propagation of Potentilla potaninii wolf.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,42(5),415-417. |
MLA | He, W. T.,et al."An efficient in vitro method for mass propagation of Potentilla potaninii wolf".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 42.5(2006):415-417. |
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