The influence of fractionation on cell survival and premature differentiation after carbon ion irradiation

doi:10.1269/jrr.08012

CORC > 近代物理研究所 > 中国科学院近代物理研究所 > 近代物理研究所知识存储（2010之前）

	The influence of fractionation on cell survival and premature differentiation after carbon ion irradiation
	Wang, Jufang1 ; Li, Renming 3; Guo, Chuanling 3; Fournier, Claudia 2; K-Weyrather, Wilma 2
刊名	JOURNAL OF RADIATION RESEARCH
	2008
卷号	49 期号:4 页码:391-398
关键词	Human-skin Fibroblasts Subcutaneous Fibrosis Heavy-ions Gamma-rays Radiotherapy Mechanisms Cultures Therapy Repair Beams
ISSN号	0449-3060
DOI	10.1269/jrr.08012
英文摘要	Carbon ion radiotherapy/Fractionated irradiation/R-BE/Premature terminal differentiation. To investigate the influence of fractionation on cell survival and radiation induced premature differentiation as markers for early and late effects after X-rays and carbon irradiation. Normal human fibroblasts NHDF, AG1522B and WI-38 were irradiated With 250 kV X-rays, or 266 MeV/u, 195 MeV/u and I I MeV/u carbon ions. Cytotoxicity was measured by a clonogenic survival assay or by determination of the differentiation pattern. Experiments with high-energy carbon ions show that fractionation induced repair effects are similar to photon irradiation. The RBE10 values for clonogenic survival are 1.3 and 1.6 for irradiation in one or two fractions for NHDF cells and around 1.2 for AG1522B cells regardless of the fractionation scheme. The RBE for a doubling of post mitotic fibroblasts (PMF) in the population is I for both single and two fractionated irradiation of NHDF cells. Using I I MeV/u carbon ions, no repair effect can be seen in WI-38 cells. The RBE10 for clonogenic survival is 3.2 for single irradiation and 4.9 for two fractionated irradiations. The RBE for a doubling of PMF is 3.1 and 5.0 for single and two fractionated irradiations, respectively. For both cell lines the effects of high-energy carbon ions representing the irradiation of the skin and the normal tissue in the entrance channel are similar to the effects of X-rays. The fractionation effects are maintained. For the lower energy, which is representative for the irradiation of the tumor region. RBE is enhanced for clonogenic survival as well as for premature terminal differentiation. Fractionation effects are not detectable. Consequently, the therapeutic ratio is significantly enhanced by fractionated irradiation with carbon ions.
WOS关键词	HUMAN-SKIN FIBROBLASTS ; SUBCUTANEOUS FIBROSIS ; HEAVY-IONS ; GAMMA-RAYS ; RADIOTHERAPY ; MECHANISMS ; CULTURES ; THERAPY ; REPAIR ; BEAMS
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000259337400007
资助机构	GSI, Germany
公开日期	2010-10-29
内容类型	期刊论文
源URL	[http://ir.imp.cas.cn/handle/113462/5617]
专题	近代物理研究所_近代物理研究所知识存储（2010之前）近代物理研究所_生物物理研究室
通讯作者	Wang, Jufang
作者单位	1.Chinese Acad Sci, IMP, Lanzhou 730000, Peoples R China 2.GSI Biophys, D-64291 Darmstadt, Germany 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jufang,Li, Renming,Guo, Chuanling,et al. The influence of fractionation on cell survival and premature differentiation after carbon ion irradiation[J]. JOURNAL OF RADIATION RESEARCH,2008,49(4):391-398.
APA	Wang, Jufang,Li, Renming,Guo, Chuanling,Fournier, Claudia,&K-Weyrather, Wilma.(2008).The influence of fractionation on cell survival and premature differentiation after carbon ion irradiation.JOURNAL OF RADIATION RESEARCH,49(4),391-398.
MLA	Wang, Jufang,et al."The influence of fractionation on cell survival and premature differentiation after carbon ion irradiation".JOURNAL OF RADIATION RESEARCH 49.4(2008):391-398.