Survivin expressions in human hepatoma HepG2 cells exposed to ionizing radiation of different LET

doi:10.1007/s00411-008-0165-0

CORC > 近代物理研究所 > 中国科学院近代物理研究所 > 近代物理研究所知识存储（2010之前）

	Survivin expressions in human hepatoma HepG2 cells exposed to ionizing radiation of different LET
	Jin, Xiao-dong1,2 ; Gong, Li1 ; Guo, Chuan-ling 1,2; Hao, Ji-fang 1; Wei, Wei1,2 ; Dai, Zhong-ying1 ; Li, Qiang1
刊名	RADIATION AND ENVIRONMENTAL BIOPHYSICS
	2008-07-01
卷号	47 期号:3 页码:399-404
ISSN号	0301-634X
DOI	10.1007/s00411-008-0165-0
英文摘要	Survivin is a member of the inhibitors of apoptosis (IAP) protein family that interferes with post-mitochondrial events including activation of caspases. To examine the regulation of survivin expression in response to irradiation with different linear energy transfer (LET), human hepatoma HepG2 cells were irradiated in vitro with X-rays and carbon ions. Cellular sensitivities to low- and high-LET radiation were determined by colony formation. Survivin expression at mRNA and protein level were measured with RT-PCR and Western blot analyses, respectively. Radiation-induced cell cycle arrest and apoptosis were investigated with flow cytometry. We found that low-LET X-rays induced dose-dependent increases in survivin expression. After exposure to high-LET carbon ions, survivin expression gradually increased from 0 to 4 Gy, and then declined at 6 Gy. More pronounced survivin expression, stronger G(2)/M phase arrest was observed after exposure to carbon ions in comparison with X-rays at doses from 0 to 4 Gy. These observations indicate that there is a differential survivin expression in response to different LET radiations and the cycle arrest mechanism may be associated with it. In addition, our data on induction of apoptosis are compatible with the assumption that survivin expression induced by low-LET X-rays radiation may play a critical role in inhibiting apoptosis. However, after irradiation with ions, an anti-apoptotic function of survivin is not evident, possibly because of the serious damage produced by densely ionizing radiation.
WOS关键词	SMMC-7721 CELLS ; APOPTOSIS ; CANCER ; RAYS ; GENE
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Biophysics ; Environmental Sciences & Ecology ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000256763500013
公开日期	2010-10-29
内容类型	期刊论文
源URL	[http://ir.imp.cas.cn/handle/113462/5605]
专题	近代物理研究所_近代物理研究所知识存储（2010之前）近代物理研究所_生物物理研究室
通讯作者	Li, Qiang
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Jin, Xiao-dong,Gong, Li,Guo, Chuan-ling,et al. Survivin expressions in human hepatoma HepG2 cells exposed to ionizing radiation of different LET[J]. RADIATION AND ENVIRONMENTAL BIOPHYSICS,2008,47(3):399-404.
APA	Jin, Xiao-dong.,Gong, Li.,Guo, Chuan-ling.,Hao, Ji-fang.,Wei, Wei.,...&Li, Qiang.(2008).Survivin expressions in human hepatoma HepG2 cells exposed to ionizing radiation of different LET.RADIATION AND ENVIRONMENTAL BIOPHYSICS,47(3),399-404.
MLA	Jin, Xiao-dong,et al."Survivin expressions in human hepatoma HepG2 cells exposed to ionizing radiation of different LET".RADIATION AND ENVIRONMENTAL BIOPHYSICS 47.3(2008):399-404.