Cell cycle and apoptosis alteration of human hepatocarcinoma cells by subclinical-dose C-12(6+)-beam irradiation

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	Cell cycle and apoptosis alteration of human hepatocarcinoma cells by subclinical-dose C-12(6+)-beam irradiation
	Zhou, Guangming1 ; Liu, Bing 1,2,3; Zhang, Hong1 ; Xie, Yi1,3 ; Hao, Jifang 1; Duan, Xin 1,3; Zhou, Qingming 1,3; Qiu, Rong 1
刊名	EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY
	2007-09-01
卷号	19 期号:9 页码:749-754
关键词	gamma-ray carbon ions cell apoptosis cell cycle hepatocellular carcinoma
ISSN号	0954-691X
英文摘要	Objective The purpose of this study is to investigate the effect of subdinical-dose C-12(6+)-beam irradiation on cell cycle and cell apoptosis in hepatocarcinoma cells. Materials and methods The HepG(2) cells were exposed to 0-2.0 Gy of either the C-12(6+) beam or a gamma-ray. Cell survival was detected by clonogenic assay. Cell cycle was determined by flow-cytometry analysis. The apoptosis was monitored by fluorescence microscope with DAPI staining. p53 and p21 expression were detected by Western blot. Results The G(0)/G(1) cells in the irradiated groups were significantly more than those in the control (P<0.05). The C-12(6+)-ion irradiation had a greater effect on the cell cycle of HepG(2) cells (including promoting G(1)-phase and G(2)-phase arrest) than gamma-ray irradiation. The apoptotic cells induced by C-12(6+) beam were significantly more numerous than those induced by gamma-ray (P<0.05). The carbon ions had a stronger effect on p53 and p21 expression than the gamma-ray irradiation. The survival fractions for cells irradiated by C-12(6+) beam were significantly smaller than those irradiated by gamma-ray (P<0.05). Conclusion The subclinical-dose C-12(6+)-beam irradiation significantly suppresses HepG(2) cells through cell-cycle arrests and cell apoptosis in contrast to same-dose gamma-ray irradiation.
WOS关键词	CARBON ION RADIOTHERAPY ; HEPATOCELLULAR-CARCINOMA ; DNA-DAMAGE ; P53 GENE ; CANCER ; THERAPY ; MUTATIONS ; PROGNOSIS ; EFFICACY ; ARREST
WOS研究方向	Gastroenterology & Hepatology
语种	英语
出版者	LIPPINCOTT WILLIAMS & WILKINS
WOS记录号	WOS:000254356300003
公开日期	2010-10-29
内容类型	期刊论文
源URL	[http://ir.imp.cas.cn/handle/113462/5479]
专题	近代物理研究所_近代物理研究所知识存储（2010之前）
通讯作者	Liu, Bing
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Lanzhou Command Ctr Dis Prevent & Control, Lanzhou, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Guangming,Liu, Bing,Zhang, Hong,et al. Cell cycle and apoptosis alteration of human hepatocarcinoma cells by subclinical-dose C-12(6+)-beam irradiation[J]. EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY,2007,19(9):749-754.
APA	Zhou, Guangming.,Liu, Bing.,Zhang, Hong.,Xie, Yi.,Hao, Jifang.,...&Qiu, Rong.(2007).Cell cycle and apoptosis alteration of human hepatocarcinoma cells by subclinical-dose C-12(6+)-beam irradiation.EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY,19(9),749-754.
MLA	Zhou, Guangming,et al."Cell cycle and apoptosis alteration of human hepatocarcinoma cells by subclinical-dose C-12(6+)-beam irradiation".EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY 19.9(2007):749-754.