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	High-efficiency transfer and expression of AdCMV-p53 in human cervix adenocarcinoma cells induced by subclinical-dose carbon beam radiation
	Liu, Bing1,2,3,4; Zhang, Hong2,4; Luo, Xiaohong1; Xie, Yi2,3,4; Hao, Jifang2; Zhou, Qingming2,3,4; Duan, Xin2,3,4; Wang, Yanling2,3,4; Zhao, Weiping2,3,4
刊名	JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
	2009-07-01
卷号	135期号:7页码:925-932
关键词	Heavy-ion beam Adenovirus vector Gene transfer Gene expression Cervix adenocarcinoma
ISSN号	0171-5216
DOI	10.1007/s00432-008-0528-6
英文摘要	The aim of this study is to evaluate the effect of carbon-beam irradiation on adenovirus-mediated p53 transfer in human cervix adenocarcinoma. The HeLa cells pre-exposed to carbon-beam or gamma-ray, were infected with replication-deficient adenovirus recombinant vectors, containing human wild-type p53 (AdCMV-p53) and green fluorescent protein (GFP) (AdCMV-GFP), respectively. The GFP transfer and p53 expression were detected by flow cytometric analysis. The GFP transfer frequency in C-beam with AdCMV-GFP groups was 38-50% more than that in gamma-ray with AdCMV-GFP groups. The percentage of p53 positive cells in the C-beam with AdCMV-p53 groups was 34-55.6% more than that in gamma-ray with AdCMV-p53 groups (p < 0.05), suggesting that subclinical-dose C-beam irradiation could significantly promote exogenous p53 transfer and p53 expression, and extend the duration of p53 expression in the HeLa cells. The expression of p21 increased with p53 expression in HeLa cells. The survival fractions for the 0.5-1.0 Gy C-beam with AdCMV-p53 groups were 38-43% less than those for the isodose gamma-ray with AdCMV-p53 groups, and 31-40% less than those for the C-beam only groups (p < 0.05). The subclinical-dose C-beam irradiation could significantly promote the transfer and expression of exogenous p53, extend the duration of p53 expression, and enhance the suppression of p53 on cervix adenocarcinoma cells.
资助项目	National Natural Science Foundation of China[10675151] ; Key Scientific Technology Research Project of Gansu Province[2GS052A43-008-02] ; Nature Science Foundation of Gansu Province[3ZS061-A25-021] ; HIRFL, National Laboratory of Heavy Ion Accelerator in Lanzhou, China
WOS关键词	WILD-TYPE P53 ; HUMAN-PAPILLOMAVIRUS TYPE-16 ; HUMAN-GENE-THERAPY ; IONIZING-RADIATION ; ION RADIOTHERAPY ; LUNG-CANCER ; MAMMALIAN-CELLS ; GLIOMA-CELLS ; ADENOVIRUS ; CARCINOMA
WOS研究方向	Oncology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000266477900008
资助机构	National Natural Science Foundation of China ; Key Scientific Technology Research Project of Gansu Province ; Nature Science Foundation of Gansu Province ; HIRFL, National Laboratory of Heavy Ion Accelerator in Lanzhou, China
公开日期	2009-11-20
内容类型	期刊论文
源URL	[http://ir.imp.cas.cn/handle/113462/174]
专题	近代物理研究所_近代物理研究所知识存储（2010之前）
通讯作者	Zhang, Hong
作者单位	1.Lanzhou Command Ctr Dis Prevent & Control, Lanzhou 730020, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 4.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Liu, Bing,Zhang, Hong,Luo, Xiaohong,et al. High-efficiency transfer and expression of AdCMV-p53 in human cervix adenocarcinoma cells induced by subclinical-dose carbon beam radiation[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2009,135(7):925-932.
APA	Liu, Bing.,Zhang, Hong.,Luo, Xiaohong.,Xie, Yi.,Hao, Jifang.,...&Zhao, Weiping.(2009).High-efficiency transfer and expression of AdCMV-p53 in human cervix adenocarcinoma cells induced by subclinical-dose carbon beam radiation.JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,135(7),925-932.
MLA	Liu, Bing,et al."High-efficiency transfer and expression of AdCMV-p53 in human cervix adenocarcinoma cells induced by subclinical-dose carbon beam radiation".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 135.7(2009):925-932.

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