Inhibiting survivin expression increases the radiosensitivity of human hepatoma HepG2 cells to high-LET carbon ions | |
Jin, X.D.1,2![]() ![]() ![]() ![]() | |
2009 | |
会议地点 | Munich, Germany |
卷号 | 25 |
期号 | 3 |
DOI | 10.1007/978-3-642-03902-7-47 |
页码 | 166-169 |
英文摘要 | In this study, we investigated whether survivin plays a direct role in mediating high-LET radiosensitivity. We designed small interfering RNA (siRNA) targeting survivin mRNA, in vitro chemo-synthesized and transfected into HepG2 human hepatoma cells. Real-time PCR assay and western bolting were used to determine survivin expressions at transcriptional and post-transcriptional levels, respectively. The survivin expressions decreased at both the transcriptional and post-transcriptional levels after treatment with survivinspecific siRNA. This observation indicates that survivinspecific siRNA could make survivin gene silent effectively. After exposure to high-LET carbon ion radiation, a reduced clonogenic survival effect was observed in cells treated with siRNA compared with those untreated with siRNA. Furthermore, Annexin V assay was used to determine apoptotic rate. Compared with siRNA mismatch and irradiation alone, siRNA treated cells showed increased apoptotic rates. We also found that transfection with survivin siRNA increased levels of G |
会议录 | World Congress on Medical Physics and Biomedical Engineering: Radiation Protection and Dosimetry, Biological Effects of Radiation
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会议录出版者 | Springer Verlag |
内容类型 | 会议论文 |
源URL | [http://119.78.100.186/handle/113462/63944] ![]() |
专题 | 中国科学院近代物理研究所 |
通讯作者 | Li, Q. |
作者单位 | 1.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China 2.Graduate School, Chinese Academy of Science, Beijing 100039, China |
推荐引用方式 GB/T 7714 | Jin, X.D.,Li, Q.,Li, P.,et al. Inhibiting survivin expression increases the radiosensitivity of human hepatoma HepG2 cells to high-LET carbon ions[C]. 见:. Munich, Germany. |
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