Inhibiting survivin expression increases the radiosensitivity of human hepatoma HepG2 cells to high-LET carbon ions

doi:10.1007/978-3-642-03902-7-47

	Inhibiting survivin expression increases the radiosensitivity of human hepatoma HepG2 cells to high-LET carbon ions
	Jin, X.D.1,2 ; Li, Q.1 ; Li, P.1; Wu, Q.F.1 ; Dai, Z.Y.1
	2009
会议地点	Munich, Germany
卷号	25
期号	3
DOI	10.1007/978-3-642-03902-7-47
页码	166-169
英文摘要	In this study, we investigated whether survivin plays a direct role in mediating high-LET radiosensitivity. We designed small interfering RNA (siRNA) targeting survivin mRNA, in vitro chemo-synthesized and transfected into HepG2 human hepatoma cells. Real-time PCR assay and western bolting were used to determine survivin expressions at transcriptional and post-transcriptional levels, respectively. The survivin expressions decreased at both the transcriptional and post-transcriptional levels after treatment with survivinspecific siRNA. This observation indicates that survivinspecific siRNA could make survivin gene silent effectively. After exposure to high-LET carbon ion radiation, a reduced clonogenic survival effect was observed in cells treated with siRNA compared with those untreated with siRNA. Furthermore, Annexin V assay was used to determine apoptotic rate. Compared with siRNA mismatch and irradiation alone, siRNA treated cells showed increased apoptotic rates. We also found that transfection with survivin siRNA increased levels of G2/M arrest. These results suggest that the radiosensitivity of HepG2 cells to high-LET carbon ions is increased after inhibiting survivin expression and gene therapy for constitutive survivin may be effective for therapeutic improvement in radioresistant solid tumors by high-LET radiation. © 2009 Springer-Verlag.
会议录	World Congress on Medical Physics and Biomedical Engineering: Radiation Protection and Dosimetry, Biological Effects of Radiation
会议录出版者	Springer Verlag
内容类型	会议论文
源URL	[http://119.78.100.186/handle/113462/63944]
专题	中国科学院近代物理研究所
通讯作者	Li, Q.
作者单位	1.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China 2.Graduate School, Chinese Academy of Science, Beijing 100039, China
推荐引用方式 GB/T 7714	Jin, X.D.,Li, Q.,Li, P.,et al. Inhibiting survivin expression increases the radiosensitivity of human hepatoma HepG2 cells to high-LET carbon ions[C]. 见:. Munich, Germany.