Heavy ion and X-ray irradiation alter the cytoskeleton and cytomechanics of cortical neurons

doi:10.4103/1673-5374.135315

	Heavy ion and X-ray irradiation alter the cytoskeleton and cytomechanics of cortical neurons
	Du, Yuting 1,2; Zhang, Jie 1,2; Zheng, Qian 1; Li, Mingxin 1; Liu, Yang 3; Zhang, Baoping 4; Liu, Bin 1,2; Zhang, Hong 3; Miao, Guoying 5
刊名	NEURAL REGENERATION RESEARCH
	2014-06-01
卷号	9 页码:1129-1137
关键词	Nerve Regeneration Radiation Brain Injury Neurons Heavy Ion X-ray Cytoskeleton Cytomechanical Properties Atomic Force Microscopy Neural Regeneration
ISSN号	1673-5374
DOI	10.4103/1673-5374.135315
文献子类	Article
英文摘要	Heavy ion beams with high linear energy transfer exhibit more beneficial physical and biological performance than conventional X-rays, thus improving the potential of this type of radiotherapy in the treatment of cancer. However, these two radiotherapy modalities both cause inevitable brain injury. The objective of this study was to evaluate the effects of heavy ion and X-ray irradiation on the cytoskeleton and cytomechanical properties of rat cortical neurons, as well as to determine the potential mechanism of neuronal injury after irradiation. Cortical neurons from 30 new-born mice were irradiated with heavy ion beams at a single dose of 2 Gy and X-rays at a single dose of 4 Gy; subsequent evaluation of their effects were carried out at 24 hours after irradiation. An immunofluorescence assay showed that after irradiation with both the heavy ion beam and X-rays, the number of primary neurons was significantly decreased, and there was evidence of apoptosis. Radiation-induced neuronal injury was more apparent after X-irradiation. Under atomic force microscopy, the neuronal membrane appeared rough and neuronal rigidity had increased. These cell changes were more apparent following exposure to X-rays. Our findings indicated that damage caused by heavy ion and X-ray irradiation resulted in the structural distortion and rearrangement of the cytoskeleton, and affected the cytomechanical properties of the cortical neurons. Moreover, this radiation injury to normal neurons was much severer after irradiation with X-rays than after heavy ion beam irradiation.
WOS关键词	ATOMIC-FORCE MICROSCOPY ; INDUCED APOPTOSIS ; NERVOUS-SYSTEM ; BRAIN ; CELLS ; DYNAMICS ; AFM ; REGENERATION ; BIOMECHANICS ; ELASTICITY
WOS研究方向	Cell Biology ; Neurosciences & Neurology
语种	英语
出版者	SHENYANG EDITORIAL DEPT NEURAL REGENERATION RES
WOS记录号	WOS:000338803900010
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/49893]
专题	中国科学院近代物理研究所
通讯作者	Liu, Bin
作者单位	1.Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Gansu, Peoples R China 2.Lanzhou Univ, Sch Nucl Sci & Technol, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Dept Radiat Biol & Med, Inst Modern Phys, Lanzhou, Gansu, Peoples R China 4.Lanzhou Univ, Sch Civil Engn & Mech, Lanzhou 730000, Gansu, Peoples R China 5.Gansu Prov Hosp, Lanzhou, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Du, Yuting,Zhang, Jie,Zheng, Qian,et al. Heavy ion and X-ray irradiation alter the cytoskeleton and cytomechanics of cortical neurons[J]. NEURAL REGENERATION RESEARCH,2014,9:1129-1137.
APA	Du, Yuting.,Zhang, Jie.,Zheng, Qian.,Li, Mingxin.,Liu, Yang.,...&Miao, Guoying.(2014).Heavy ion and X-ray irradiation alter the cytoskeleton and cytomechanics of cortical neurons.NEURAL REGENERATION RESEARCH,9,1129-1137.
MLA	Du, Yuting,et al."Heavy ion and X-ray irradiation alter the cytoskeleton and cytomechanics of cortical neurons".NEURAL REGENERATION RESEARCH 9(2014):1129-1137.