Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes

doi:10.1016/j.toxlet.2014.01.001

	Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes
	Li, Hongyan 1,2,3,4; He, Yuxuan 5; Zhang, Hong1,2,3 ; Miao, Guoying 1,4,6
刊名	TOXICOLOGY LETTERS
	2014-03-21
卷号	225 页码:433-444
关键词	Proteome Pubertal Mice Testis Carbon Ion radiatIon
ISSN号	0378-4274
DOI	10.1016/j.toxlet.2014.01.001
文献子类	Article
英文摘要	Heavy ion radiation, a high linear energy transfer (LET) radiation, has been shown to have adverse effects on reproduction in male mice. The aim of this study was to profile and investigate the differentially expressed proteins in pubertal male mice testes following carbon ion radiation (CIR). Male mice underwent whole-body irradiation with CIR (1 and 4 Gy), and MALDI-TOF/TOF analysis was used to investigate the alteration in protein expression in 2-DE (two-dimensional gel electrophoresis) gels of testes caused by irradiation after 14 days. 8 differentially expressed proteins were identified and these proteins were mainly involved in energy supply, the endoplasmic reticulum, cell proliferation, cell cycle, antioxidant capacity and mitochondrial respiration, which play important roles in the inhibition of testicular function in response to CIR. Furthermore, we confirmed the relationship between transcription of mRNA and the abundance of proteins. Our results indicated that these proteins may lead to new insights into the molecular mechanism of CIR toxicity, and suggested that the gene expression response to CIR involves diverse regulatory mechanisms from transcription of mRNA to the formation of functional proteins. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
资助项目	Fostering Foundation for the Excellent Ph.D. Dissertation of Gansu Agricultural University[2013002]
WOS关键词	FOLLICLE-STIMULATING-HORMONE ; SPERMATOGENIC CELL APOPTOSIS ; LEYDIG-CELL ; TESTICULAR STEROIDOGENESIS ; PERFLUORODODECANOIC ACID ; ENDOPLASMIC-RETICULUM ; RECEPTOR-ALPHA ; FREE-RADICALS ; HEAT-STRESS ; RADIATION
WOS研究方向	Toxicology
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000331725400013
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/49809]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Hong
作者单位	1.Chinese Acad Sci, Dept Heavy Ion Radiat Med, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China 6.Gansu Prov Hosp, Dept Radiotherapy, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Li, Hongyan,He, Yuxuan,Zhang, Hong,et al. Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes[J]. TOXICOLOGY LETTERS,2014,225:433-444.
APA	Li, Hongyan,He, Yuxuan,Zhang, Hong,&Miao, Guoying.(2014).Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes.TOXICOLOGY LETTERS,225,433-444.
MLA	Li, Hongyan,et al."Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes".TOXICOLOGY LETTERS 225(2014):433-444.