Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes | |
Li, Hongyan1,2,3,4; He, Yuxuan5; Zhang, Hong1,2,3![]() | |
刊名 | TOXICOLOGY LETTERS
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2014-03-21 | |
卷号 | 225页码:433-444 |
关键词 | Proteome Pubertal Mice Testis Carbon Ion radiatIon |
ISSN号 | 0378-4274 |
DOI | 10.1016/j.toxlet.2014.01.001 |
文献子类 | Article |
英文摘要 | Heavy ion radiation, a high linear energy transfer (LET) radiation, has been shown to have adverse effects on reproduction in male mice. The aim of this study was to profile and investigate the differentially expressed proteins in pubertal male mice testes following carbon ion radiation (CIR). Male mice underwent whole-body irradiation with CIR (1 and 4 Gy), and MALDI-TOF/TOF analysis was used to investigate the alteration in protein expression in 2-DE (two-dimensional gel electrophoresis) gels of testes caused by irradiation after 14 days. 8 differentially expressed proteins were identified and these proteins were mainly involved in energy supply, the endoplasmic reticulum, cell proliferation, cell cycle, antioxidant capacity and mitochondrial respiration, which play important roles in the inhibition of testicular function in response to CIR. Furthermore, we confirmed the relationship between transcription of mRNA and the abundance of proteins. Our results indicated that these proteins may lead to new insights into the molecular mechanism of CIR toxicity, and suggested that the gene expression response to CIR involves diverse regulatory mechanisms from transcription of mRNA to the formation of functional proteins. (C) 2014 Elsevier Ireland Ltd. All rights reserved. |
资助项目 | Fostering Foundation for the Excellent Ph.D. Dissertation of Gansu Agricultural University[2013002] |
WOS关键词 | FOLLICLE-STIMULATING-HORMONE ; SPERMATOGENIC CELL APOPTOSIS ; LEYDIG-CELL ; TESTICULAR STEROIDOGENESIS ; PERFLUORODODECANOIC ACID ; ENDOPLASMIC-RETICULUM ; RECEPTOR-ALPHA ; FREE-RADICALS ; HEAT-STRESS ; RADIATION |
WOS研究方向 | Toxicology |
语种 | 英语 |
出版者 | ELSEVIER IRELAND LTD |
WOS记录号 | WOS:000331725400013 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.186/handle/113462/49809] ![]() |
专题 | 中国科学院近代物理研究所 |
通讯作者 | Zhang, Hong |
作者单位 | 1.Chinese Acad Sci, Dept Heavy Ion Radiat Med, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China 6.Gansu Prov Hosp, Dept Radiotherapy, Lanzhou 730000, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Hongyan,He, Yuxuan,Zhang, Hong,et al. Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes[J]. TOXICOLOGY LETTERS,2014,225:433-444. |
APA | Li, Hongyan,He, Yuxuan,Zhang, Hong,&Miao, Guoying.(2014).Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes.TOXICOLOGY LETTERS,225,433-444. |
MLA | Li, Hongyan,et al."Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes".TOXICOLOGY LETTERS 225(2014):433-444. |
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