Identification of Serum microRNA Biomarkers for Tuberculosis Using RNA-seq

doi:10.1371/journal.pone.0088909

	Identification of Serum microRNA Biomarkers for Tuberculosis Using RNA-seq
	Zhang, Hongtai 1,2,3; Sun, Zhaogang 4; Wei, Wenjing 2; Liu, Zhonghui 5; Fleming, Joy 2; Zhang, Shuai 2; Lin, Nan 6; Wang, Ming 2; Chen, Maoshan 7; Xu, Yuhui 4
刊名	PLOS ONE
	2014-02-20
卷号	9
ISSN号	1932-6203
DOI	10.1371/journal.pone.0088909
文献子类	Article
英文摘要	Tuberculosis (TB) remains a significant human health issue. More effective biomarkers for use in tuberculosis prevention, diagnosis, and treatment, including markers that can discriminate between healthy individuals and those with latent infection, are urgently needed. To identify a set of such markers, we used Solexa sequencing to examine microRNA expression in the serum of patients with active disease, healthy individuals with latent TB, and those with or without prior BCG inoculation. We identified 24 microRNAs that are up-regulated (2.85-1285.93 fold) and 6 microRNAs that are down-regulated (0.003-0.11 fold) (P < 0.05) in patients with active TB relative to the three groups of healthy controls. In addition, 75 microRNAs were up-regulated (2.05-2454.58 fold) and 11 were down-regulated (0.001-0.42 fold) (P < 0.05) in latent-TB infected individuals relative to BCG-inoculated individuals. Of interest, 134 microRNAs were differentially-expressed in BCG-inoculated relative to un-inoculated individuals (18 up-regulated 2.9-499.29 fold, 116 down-regulated 0.0002-0.5 fold), providing insights into the effects of BCG inoculation at the microRNA level. Target prediction of differentially-expressed microRNAs by microRNA-Gene Network analysis and analysis of pathways affected suggest that regulation of the host immune system by microRNAs is likely to be one of the main factors in the pathogenesis of tuberculosis. qRT-PCR validation indicated that hsa-miR-196b and hsa-miR-376c have potential as markers for active TB disease. The microRNA differential-expression profiles generated in this study provide a good foundation for the development of markers for TB diagnosis, and for investigations on the role of microRNAs in BCG-inoculated and latent-infected individuals.
资助项目	Key Project Specialized for Infectious Diseases of the Chinese Ministry of Health[2012ZX10003002] ; Key Project Specialized for Infectious Diseases of the Chinese Ministry of Health[2013ZX10003006]
WOS关键词	MYCOBACTERIUM-TUBERCULOSIS ; MESSENGER-RNA ; EXPRESSION ; INFECTION ; CELLS ; NFAT5 ; DIAGNOSIS ; TISSUES ; CANCER ; ARRAY
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000331714700041
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/49745]
专题	中国科学院近代物理研究所
通讯作者	Li, Chuanyou
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Dept Space Radiobiol, Lanzhou, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Key Lab Noncoding RNA, Beijing 100080, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Capital Med Univ, Beijing Chest Hosp, Beijing Key Lab Drug Resistance TB, Beijing, Peoples R China 5.Huazhong Agr Univ, Wuhan, Hubei, Peoples R China 6.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou, Peoples R China 7.Beijing Genom Inst, Shenzhen 518083, Peoples R China 8.4th Peoples Hosp, Foshan, Guangdong, Peoples R China 9.Capital Med Univ, Beijing Chest Hosp, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Hongtai,Sun, Zhaogang,Wei, Wenjing,et al. Identification of Serum microRNA Biomarkers for Tuberculosis Using RNA-seq[J]. PLOS ONE,2014,9.
APA	Zhang, Hongtai.,Sun, Zhaogang.,Wei, Wenjing.,Liu, Zhonghui.,Fleming, Joy.,...&Zhou, Guangming.(2014).Identification of Serum microRNA Biomarkers for Tuberculosis Using RNA-seq.PLOS ONE,9.
MLA	Zhang, Hongtai,et al."Identification of Serum microRNA Biomarkers for Tuberculosis Using RNA-seq".PLOS ONE 9(2014).