Salidroside inhibits migration and invasion of human fibrosarcoma HT1080 cells

doi:10.1016/j.phymed.2011.09.070

	Salidroside inhibits migration and invasion of human fibrosarcoma HT1080 cells
	Sun, Chao 1,2,3; Wang, Zhenhua1,2,4 ; Zheng, Qiusheng 4; Zhang, Hong1,2
刊名	PHYTOMEDICINE
	2012-02-15
卷号	19 页码:355-363
关键词	Salidroside Invasion Metastasis Ht1080 Cells Reactive Oxygen Species
ISSN号	0944-7113
DOI	10.1016/j.phymed.2011.09.070
文献子类	Article
英文摘要	Oxidative stress plays an important role in tumorigenesis and metastasis. Salidroside, a phenylpropanoid glycoside isolated from Rhodiola rosea L. shows potent antioxidant property. Here we investigated the inhibitory effects of salidroside on tumor metastasis in human fibrosarcoma HT1080 cells in vitro. The results indicated that salidroside significantly reduced wound closure areas of HT1080 cells, inhibited HT1080 cells invasion into Matrigel-coated membranes, suppressed matrix metalloproteinases (MMP-2 and MMP-9) activity, and increased tissue inhibitor of metalloproteinase-2 (TIMP-2) expression in a dose-dependent manner in HT1080 cells. Salidroside treatment upregulated the E-cadherin expression, while downregulated the expression of beta 1-integrin. As an antioxidant, salidroside inhibited the intracellular reactive oxygen species (ROS) formation in a dose-dependent manner. The results also showed that salidroside could inhibit the activation of protein kinase C (PKC) and the phosphorylation of extracellular signal-regulated kinase 1 and 2 (ERK1/2) in a dose-dependent manner. In conclusion, these results suggest that salidroside inhibits tumor cells metastasis, which may due to its interfere in the intracellular excess ROS thereby down-regulated the ROS-PKC-ERK1/2 signaling pathway. (C) 2011 Elsevier GmbH. All rights reserved.
资助项目	China Postdoctoral Science Foundation[200902313]
WOS关键词	SIGNAL-REGULATED KINASE ; CANCER METASTASIS ; OXIDATIVE STRESS ; INTEGRIN EXPRESSION ; ADHESION MOLECULES ; HYDROGEN-PEROXIDE ; DEPENDENT PATHWAY ; TUMOR PROGRESSION ; INDUCED APOPTOSIS ; EPITHELIAL-CELLS
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	ELSEVIER GMBH, URBAN & FISCHER VERLAG
WOS记录号	WOS:000302928400025
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/48797]
专题	中国科学院近代物理研究所
通讯作者	Wang, Zhenhua
作者单位	1.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 4.Shihezi Univ, Key Lab Xinjiang Endem Phytomed Resources, Minist Educ, Sch Pharm, Shihezi, Peoples R China
推荐引用方式 GB/T 7714	Sun, Chao,Wang, Zhenhua,Zheng, Qiusheng,et al. Salidroside inhibits migration and invasion of human fibrosarcoma HT1080 cells[J]. PHYTOMEDICINE,2012,19:355-363.
APA	Sun, Chao,Wang, Zhenhua,Zheng, Qiusheng,&Zhang, Hong.(2012).Salidroside inhibits migration and invasion of human fibrosarcoma HT1080 cells.PHYTOMEDICINE,19,355-363.
MLA	Sun, Chao,et al."Salidroside inhibits migration and invasion of human fibrosarcoma HT1080 cells".PHYTOMEDICINE 19(2012):355-363.