Biological characteristics of Rh123(high) stem-like cells in a side population of 786-O renal carcinoma cells

doi:10.3892/ol.2013.1270

	Biological characteristics of Rh123(high) stem-like cells in a side population of 786-O renal carcinoma cells
	Lu, Jianzhong 1; Cui, Yong 1; Zhu, Jiayun2 ; He, Jinpeng2 ; Zhou, Guangming2 ; Yue, Zhongjin 1
刊名	ONCOLOGY LETTERS
	2013-06-01
卷号	5 页码:1903-1908
关键词	Rhodamine 123 Cancer Stem Cell Renal Cell Carcinoma Flow Cytometery Tumorigenesis
ISSN号	1792-1074
DOI	10.3892/ol.2013.1270
文献子类	Article
英文摘要	The aim of the present study was to investigate the differences in biological characteristics between the rhodamine 123 (Rh123)(high) and Rh123(low) subpopulations of the renal cancer cell line 786-O and to identify evidence for the existence of cancer stem cells in renal cell carcinoma (RCC) cells. In vitro cultured RCC 786-O cells were stained with Rh123, analyzed and sorted with flow cytometry. The differences in proliferative activity, long-term differentiation and radiation sensitivity between the two subpopulations were measured and the oncogenicity of each subpopulation was evaluated according to their neoplastic growth ability in soft agar and tumor-forming ability in NOD/SCID immunodeficient mice. There were two subpopulations in the cultured 786-O cells, Rh123(high) and Rh123(low) cells. Rh123(low) cells were the majority among 786-O renal carcinoma cells and barely formed solid tumors in NOD/SCID mice and colonies in soft agar. By contrast, the Rh123(high) cells were the minority, exhibited high proliferative activity, differentiation ability and resistance to radiation and showed high tumorigenesis potential and colony forming efficiency. The Rh123(high) cells had stem-like characteristics in cultured RCC 786-O cells in vitro.
资助项目	National Natural Science Foundation of China[30571860]
WOS关键词	PROSPECTIVE IDENTIFICATION
WOS研究方向	Oncology
语种	英语
出版者	SPANDIDOS PUBL LTD
WOS记录号	WOS:000324816500029
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/48351]
专题	中国科学院近代物理研究所
通讯作者	Yue, Zhongjin
作者单位	1.Lanzhou Univ, Hosp 2, Key Lab Urol Dis Gansu Prov, Inst Urol,Gansu Nephrourol Clin Ctr, Lanzhou 730030, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Radiobiol Effects Grp, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Lu, Jianzhong,Cui, Yong,Zhu, Jiayun,et al. Biological characteristics of Rh123(high) stem-like cells in a side population of 786-O renal carcinoma cells[J]. ONCOLOGY LETTERS,2013,5:1903-1908.
APA	Lu, Jianzhong,Cui, Yong,Zhu, Jiayun,He, Jinpeng,Zhou, Guangming,&Yue, Zhongjin.(2013).Biological characteristics of Rh123(high) stem-like cells in a side population of 786-O renal carcinoma cells.ONCOLOGY LETTERS,5,1903-1908.
MLA	Lu, Jianzhong,et al."Biological characteristics of Rh123(high) stem-like cells in a side population of 786-O renal carcinoma cells".ONCOLOGY LETTERS 5(2013):1903-1908.