Effects of carbon-ion beam or X-ray irradiation on anti-apoptosis Delta Np73 expression in HeLa cells

doi:10.1016/j.gene.2012.11.040

	Effects of carbon-ion beam or X-ray irradiation on anti-apoptosis Delta Np73 expression in HeLa cells
	Di, Cui-Xia1,3,4 ; Yang, Li-Na 2; Zhang, Hong1,2,3 ; An, Li-Zhe 2; Zhang, Xin1,2,3 ; Ma, Xiao-Fei 1,2,3; Sun, Chao 1,2,3; Wang, Xiao-Hu 5; Yang, Rong 6; Wu, Zhen-hua1,3,4
刊名	GENE
	2013-02-15
卷号	515 页码:208-213
关键词	Carbon-ion Beam X-ray Apoptosis Cervical Carcinoma Delta Np73
ISSN号	0378-1119
DOI	10.1016/j.gene.2012.11.040
文献子类	Article
英文摘要	Delta Np73 has emerged as an interesting novel factor in cancer research. Here, we report the effect of carbon-ion beams on Delta Np73 expression in human cervix carcinoma HeLa cells in contrast to the effect of X-rays. Cellular sensitivities were determined by colony formation. Radiation-induced cell cycle arrest was investigated with flow cytometry. Additionally, radiation-induced apoptosis was analyzed with flow cytometry and Hoechst staining. Furthermore, Delta Np73 expression was examined by semi-quantitative reverse transcription-PCR (semi-quantitative RT-PCR) as well as by Western blot analysis. Following irradiation, stronger G2/M phase arrest, more significant increase in apoptosis and more pronounced Delta Np73 degradation were observed after exposure to high-LET carbon beams in comparison with X-rays at 4 Gy doses. These observations indicate that there is a differential Delta Np73 expression in response to different LET radiations, and down-regulated Delta Np73 expression might play a critical role in promoting cycle arrest and apoptosis in cancer cells. This study highlights the potential of Delta Np73 in radiotherapy. (C) 2012 Elsevier B.V. All rights reserved.
资助项目	Western Talent Program of Chinese Academy of Sciences[Y260230XB0]
WOS关键词	CERVICAL-CANCER CELLS ; HIGH-LET RADIATION ; IN-VIVO ; POOR-PROGNOSIS ; OVARIAN-CANCER ; LUNG-CANCER ; THERAPY ; P53 ; RADIOTHERAPY ; INDUCTION
WOS研究方向	Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000314559400030
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/48041]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Hong
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Dept Heavy Ion Radiat Med, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Sch Life Sci, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 4.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 5.Gansu Prov Canc Hosp, Dept Radiotherapy, Lanzhou 730050, Peoples R China 6.Gansu Prov Canc Hosp, Dept Pathol, Lanzhou 730050, Peoples R China
推荐引用方式 GB/T 7714	Di, Cui-Xia,Yang, Li-Na,Zhang, Hong,et al. Effects of carbon-ion beam or X-ray irradiation on anti-apoptosis Delta Np73 expression in HeLa cells[J]. GENE,2013,515:208-213.
APA	Di, Cui-Xia.,Yang, Li-Na.,Zhang, Hong.,An, Li-Zhe.,Zhang, Xin.,...&Si, Jing.(2013).Effects of carbon-ion beam or X-ray irradiation on anti-apoptosis Delta Np73 expression in HeLa cells.GENE,515,208-213.
MLA	Di, Cui-Xia,et al."Effects of carbon-ion beam or X-ray irradiation on anti-apoptosis Delta Np73 expression in HeLa cells".GENE 515(2013):208-213.