Proteomic analysis for testis of Mice exposed to carbon ion radiation

doi:10.1016/j.mrgentox.2013.06.017

	Proteomic analysis for testis of Mice exposed to carbon ion radiation
	Li, Hongyan 1,2,3,4; Zhang, Hong1,2,3 ; Xie, Yi1,2,3 ; He, Yuxuan 5; Miao, Guoying 1,2,3,4; Yang, Lina 1; Di, Cuixia1,2,3 ; He, Yang 1,2,3,4
刊名	MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
	2013-08-15
卷号	755 页码:148-155
关键词	Proteomics Testis Carbon Ion radiatIon
ISSN号	1383-5718
DOI	10.1016/j.mrgentox.2013.06.017
文献子类	Article
英文摘要	This paper investigates the mechanism of action of heavy ion radiation (HIR) on mouse testes. The testes of male mice subjected to whole body irradiation with carbon ion beam (0.5 and 4Gy) were analyzed at 7 days after irradiation. A two-dimensional gel electrophoresis approach was employed to investigate the alteration of protein expression in the testes. Spot detection and matching were performed using the PDQuest 8.0 software. A difference of more than threefold in protein quantity (normalized spot volume) is the standard for detecting differentially expressed protein spots. A total of II differentially expressed proteins were found. Protein identification was performed using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF). Nine specific proteins were identified by searching the protein sequence database of the National Center for Biotechnology Information. These proteins were found involved in molecular chaperones, metabolic enzymes, oxidative stress, sperm function, and spermatogenic cell proliferation. HIR decreased glutathione activity and increased malondialdehyde content in the testes. Given that Pin1 is related to the cell cycle and that proliferation is affected by spermatogenesis, we analyzed testicular histological changes and Pin1 protein expression through immunoblotting and immunofluorescence. Alterations of multiple pathways may be associated with HIR toxicity to the testes. Our findings are essential for studies on the development, biology, and pathology of Mouse testes after HIR in space or radiotherapy. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.
资助项目	Scientific Technology Research Projects of Gansu Province[0702NKDA045] ; Scientific Technology Research Projects of Gansu Province[0806RJYA020]
WOS关键词	OXIDATIVE STRESS ; CELL-CYCLE ; ADAPTIVE RESPONSE ; INDUCED APOPTOSIS ; DNA-DAMAGE ; RAT TESTIS ; PROTEIN ; EXPRESSION ; MOUSE ; SPERMATOGENESIS
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000323630100010
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/48031]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Hong
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Dept Heavy Ion Radiat Med, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China
推荐引用方式 GB/T 7714	Li, Hongyan,Zhang, Hong,Xie, Yi,et al. Proteomic analysis for testis of Mice exposed to carbon ion radiation[J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,2013,755:148-155.
APA	Li, Hongyan.,Zhang, Hong.,Xie, Yi.,He, Yuxuan.,Miao, Guoying.,...&He, Yang.(2013).Proteomic analysis for testis of Mice exposed to carbon ion radiation.MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,755,148-155.
MLA	Li, Hongyan,et al."Proteomic analysis for testis of Mice exposed to carbon ion radiation".MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 755(2013):148-155.