Personalised proteome analysis by means of protein microarrays made from individual patient samples

doi:10.1038/srep39756

	Personalised proteome analysis by means of protein microarrays made from individual patient samples
	Syafrizayanti 1,2; Lueong, Smiths S.1; Di, Cuixia 1,3; Schaefer, Jonas V.4; Pluckthun, Andreas 4; Hoheisel, Joerg D.1
刊名	SCIENTIFIC REPORTS
	2017-01-03
卷号	7 页码:7
ISSN号	2045-2322
DOI	10.1038/srep39756
英文摘要	DNA sequencing has advanced to a state that permits studying the genomes of individual patients as nearly a matter of routine. Towards analysing a tissue's protein content in a similar manner, we established a method for the production of microarrays that represent full-length proteins as they are encoded in individual specimens, exhibiting the particular variations, such as mutations or splice variations, present in these samples. From total RNA isolates, each transcript is copied to a specific location on the array by an on-chip polymerase elongation reaction, followed by in situ cell-free transcription and translation. These microarrays permit parallel analyses of variations in protein structure and interaction that are specific to particular samples.
资助项目	European Union, AffinityProteome ; European Union, Affinomics consortia ; PaCaNet project - German Federal Ministry of Education and Research (BMBF) ; Directorate General of Higher Education (DGHE) of the Republic of Indonesia ; Deutsche Akademischer Austauschdienst (DAAD)
WOS关键词	ANTIBODY MICROARRAYS ; EXPRESSION ; ARRAYS ; GENERATION ; DRAFT ; DNA
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000391030500001
资助机构	European Union, AffinityProteome ; European Union, Affinomics consortia ; PaCaNet project - German Federal Ministry of Education and Research (BMBF) ; Directorate General of Higher Education (DGHE) of the Republic of Indonesia ; Deutsche Akademischer Austauschdienst (DAAD)
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/44073]
专题	中国科学院近代物理研究所
通讯作者	Hoheisel, Joerg D.
作者单位	1.Deutsch Krebsforschungszentrum DKFZ, Div Funct Genome Anal, Neuenheimer Feld 580, D-69120 Heidelberg, Germany 2.Andalas Univ, Fac Math & Nat Sci, Dept Chem, Kampus Limau Manis, Padang, Indonesia 3.Chinese Acad Sci, Key Lab Heavy Ion Radiat Med Gansu Prov, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Peoples R China 4.Univ Zurich, Dept Biochem, Winterthurerstr 190, CH-8057 Zurich, Switzerland
推荐引用方式 GB/T 7714	Syafrizayanti,Lueong, Smiths S.,Di, Cuixia,et al. Personalised proteome analysis by means of protein microarrays made from individual patient samples[J]. SCIENTIFIC REPORTS,2017,7:7.
APA	Syafrizayanti,Lueong, Smiths S.,Di, Cuixia,Schaefer, Jonas V.,Pluckthun, Andreas,&Hoheisel, Joerg D..(2017).Personalised proteome analysis by means of protein microarrays made from individual patient samples.SCIENTIFIC REPORTS,7,7.
MLA	Syafrizayanti,et al."Personalised proteome analysis by means of protein microarrays made from individual patient samples".SCIENTIFIC REPORTS 7(2017):7.