Comparative proteomics reveals the underlying toxicological mechanism of low sperm motility induced by iron ion radiation in mice

doi:10.1016/j.reprotox.2016.07.014

	Comparative proteomics reveals the underlying toxicological mechanism of low sperm motility induced by iron ion radiation in mice
	Li, Hongyan 1,2,3; He, Yuxuan 4; Yan, Jiawei 1,2,3,5; Zhao, Qiuyue 1,2,3; Di, Cuixia1,2,3 ; Zhang, Hong1,2,3
刊名	REPRODUCTIVE TOXICOLOGY
	2016-10-01
卷号	65 页码:148-158
关键词	Proteomics Sperm Motility Space radiation
ISSN号	0890-6238
DOI	10.1016/j.reprotox.2016.07.014
英文摘要	The toxicological mechanism of low sperm motility induced by iron ion radiation (IIR) was investigated in mice. Reproductive organ indices were measured following whole-body irradiation with a 2 Gy iron ion beam. Two-dimensional gel electrophoresis, immunoblotting and immunofluorescence were used to analyze protein expression, and real-time polymerase chain reaction was performed to confirm altered gene expression. Reproductive organ indices and sperm motility were lowest 2 weeks after IIR. Sperm function changes via testis and cauda epididymis function were also determined at this time point. Sixteen differentially expressed proteins were identified in sperm 2 weeks after IIR. Bioinformatics analysis indicated that alpha-enolase (Enol) may be important in the regulation of glycolysis in sperm, and Enol expression was correlated with sperm motility. Enol may be a potential marker for low sperm motility induced by IIR, and these results may provide a useful reference for changes in astronaut fertility during long space missions. (C) 2016 Elsevier Inc. All rights reserved.
资助项目	National Natural Science Foundation of China[Y660010GJ0] ; National Natural Science Foundation of China[U1432248] ; Western Talent Program of the Chinese Academy of Sciences[Y560020XB0] ; Ministry of Science and Technology National Key RD project[2016YFC0904600] ; Fostering Foundation for the Excellent Ph.D. Dissertation of Gansu Agricultural University[2013002]
WOS关键词	CREATINE-KINASE ; GFAP EXPRESSION ; SPACE RADIATION ; MOUSE TESTIS ; CELL-LINE ; IN-VITRO ; SPERMATOZOA ; MEN ; INFERTILITY ; IRRADIATION
WOS研究方向	Reproductive Biology ; Toxicology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000385990900018
资助机构	National Natural Science Foundation of China ; Western Talent Program of the Chinese Academy of Sciences ; Ministry of Science and Technology National Key RD project ; Fostering Foundation for the Excellent Ph.D. Dissertation of Gansu Agricultural University
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/43571]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Hong
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Dept Radiat Med, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou 730000, Peoples R China 4.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Hongyan,He, Yuxuan,Yan, Jiawei,et al. Comparative proteomics reveals the underlying toxicological mechanism of low sperm motility induced by iron ion radiation in mice[J]. REPRODUCTIVE TOXICOLOGY,2016,65:148-158.
APA	Li, Hongyan,He, Yuxuan,Yan, Jiawei,Zhao, Qiuyue,Di, Cuixia,&Zhang, Hong.(2016).Comparative proteomics reveals the underlying toxicological mechanism of low sperm motility induced by iron ion radiation in mice.REPRODUCTIVE TOXICOLOGY,65,148-158.
MLA	Li, Hongyan,et al."Comparative proteomics reveals the underlying toxicological mechanism of low sperm motility induced by iron ion radiation in mice".REPRODUCTIVE TOXICOLOGY 65(2016):148-158.