MicroRNA-449a enhances radiosensitivity by downregulation of c-Myc in prostate cancer cells

doi:10.1038/srep27346

	MicroRNA-449a enhances radiosensitivity by downregulation of c-Myc in prostate cancer cells
	Mao, Aihong 1,2,3,4; Zhao, Qiuyue 1,4,5,6; Zhou, Xin1,5,6 ; Sun, Chao 1,5,6; Si, Jing1,5,6 ; Zhou, Rong 1,5,6; Gan, Lu1,5,6 ; Zhang, Hong1,5,6
刊名	SCIENTIFIC REPORTS
	2016-06-02
卷号	6 页码:13
ISSN号	2045-2322
DOI	10.1038/srep27346
英文摘要	MicroRNAs (miRNAs) have been reported to be involved in DNA damage response induced by ionizing radiation (IR). c-Myc is reduced when cells treated with IR or other DNA damaging agents. It is unknown whether miRNAs participate in c-Myc downregulation in response to IR. In the present study, we found that miR-449a enhanced radiosensitivity in vitro and in vivo by targeting c-Myc in prostate cancer (LNCaP) cells. MiR-449a was upregulated and c-Myc was downregulated in response to IR in LNCaP cells. Overexpression of miR-449a or knockdown of c-Myc promoted the sensitivity of LNCaP cells to IR. By establishing c-Myc as a direct target of miR-449a, we revealed that miR-449a enhanced radiosensitivity by repressing c-Myc expression in LNCaP cells. Furthermore, we showed that miR-449a enhanced radiation-induced G2/M phase arrest by directly downregulating c-Myc, which controlled the Cdc2/CyclinB1 cell cycle signal by modulating Cdc25A. These results highlight an unrecognized mechanism of miR-449a-mediated c-Myc regulation in response to IR and may provide alternative therapeutic strategies for the treatment of prostate cancer.
资助项目	Key program of National Natural Science Foundation of China[U1432248] ; National Natural Science Foundation of China[11305226] ; National Natural Science Foundation of China[11405230] ; National Natural Science Foundation of China[11505245] ; Scientific Technology Research Projects of Gansu Province[1107RJYA033]
WOS关键词	TUMOR-SUPPRESSOR ; CLINICAL-SIGNIFICANCE ; IONIZING-RADIATION ; GASTRIC-CANCER ; UP-REGULATION ; EXPRESSION ; ONCOGENE ; TARGET ; GROWTH ; CYCLE
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000376862000001
资助机构	Key program of National Natural Science Foundation of China ; National Natural Science Foundation of China ; Scientific Technology Research Projects of Gansu Province
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/42679]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Hong
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, Sch Nucl Sci & Technol, Lanzhou 730000, Peoples R China 3.Inst Gansu Med Sci Res, Lanzhou 730050, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 5.Chinese Acad Sci, Key Lab Heavy Ion Radiat Med, Lanzhou 730000, Peoples R China 6.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Mao, Aihong,Zhao, Qiuyue,Zhou, Xin,et al. MicroRNA-449a enhances radiosensitivity by downregulation of c-Myc in prostate cancer cells[J]. SCIENTIFIC REPORTS,2016,6:13.
APA	Mao, Aihong.,Zhao, Qiuyue.,Zhou, Xin.,Sun, Chao.,Si, Jing.,...&Zhang, Hong.(2016).MicroRNA-449a enhances radiosensitivity by downregulation of c-Myc in prostate cancer cells.SCIENTIFIC REPORTS,6,13.
MLA	Mao, Aihong,et al."MicroRNA-449a enhances radiosensitivity by downregulation of c-Myc in prostate cancer cells".SCIENTIFIC REPORTS 6(2016):13.