Cloning and expression analysis of a Toll-like receptor 22 (tlr22) gene from turbot, Scophthalmus maximus | |
Hu, Guo-Bin1,2; Zhang, Shou-Feng1; Yang, Xi1; Liu, Da-Hai3; Liu, Qiu-Ming1; Zhang, Shi-Cui1,2 | |
刊名 | FISH & SHELLFISH IMMUNOLOGY
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2015-06 | |
卷号 | 44期号:2页码:399-409 |
关键词 | Scophthalmus maximus Tlr22 Structural characteristics Gene expression PAMPs |
ISSN号 | 1050-4648 |
DOI | 10.1016/j.fsi.2015.03.001 |
英文摘要 | Toll-like receptor 22 (TLR22) exists exclusively in aquatic animals and recognizes double stranded RNA (dsRNA). In the present study, a tlr22 gene and its 5'-flanking sequence were cloned from turbot, Scophthalmus maximus, its immune responsive expression was subsequently studied in vivo. The turbot (sm)tlr22 gene spans over 5.6 kb with a structure of 4 exon-3 intron and encodes 962 amino acids. The deduced protein shows the highest sequence identity (76.7%) to Japanese flounder Tlr22 and possesses a signal peptide sequence, a leucine-rich repeat (LRR) domain composed of 27 LRR motifs, a transmembrane region and a Toll/interleukin-1 receptor (TIR) domain. Phylogenetic analysis grouped it with other teleost Tlr22s. The interferon-stimulated response element (ISRE) and signal transducer and activator of transcription (STAT) binding site important for the basal transcriptional activity of TLR3 were predicted in the 5'-flanking sequence of smtlr22 gene. Quantitative real-time PCR (qPCR) analysis demonstrated the constitutive expression of smtlr22 mRNA in all examined tissues with higher levels in the head kidney, kidney and spleen. Further, smtlr22 expression was significantly up-regulated following challenge with polyinosinic: polycytidylic acid (poly I:C), lipopolysaccharide (LPS) or turbot reddish body iridovirus (TRBIV) in the gills, head kidney, spleen and muscle, with maximum increases ranging from 2.56 to 6.24 fold upon different immunostimulants and organs. These findings suggest a possible role of Smtlr22 in the immune responses to the infections of a broad range of pathogens that include DNA and RNA viruses and Gram-negative bacteria. (c) 2015 Elsevier Ltd. All rights reserved. |
资助项目 | Shandong Provincial Natural Science Foundation[ZR2013CM045] |
WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
语种 | 英语 |
出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
WOS记录号 | WOS:000354583800002 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn/handle/2SI8HI0U/3660] ![]() |
专题 | 业务部门_海洋政策研究中心 |
作者单位 | 1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China; 2.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Peoples R China; 3.State Ocean Adm China, Inst Oceanog 1, Qingdao 266061, Peoples R China |
推荐引用方式 GB/T 7714 | Hu, Guo-Bin,Zhang, Shou-Feng,Yang, Xi,et al. Cloning and expression analysis of a Toll-like receptor 22 (tlr22) gene from turbot, Scophthalmus maximus[J]. FISH & SHELLFISH IMMUNOLOGY,2015,44(2):399-409. |
APA | Hu, Guo-Bin,Zhang, Shou-Feng,Yang, Xi,Liu, Da-Hai,Liu, Qiu-Ming,&Zhang, Shi-Cui.(2015).Cloning and expression analysis of a Toll-like receptor 22 (tlr22) gene from turbot, Scophthalmus maximus.FISH & SHELLFISH IMMUNOLOGY,44(2),399-409. |
MLA | Hu, Guo-Bin,et al."Cloning and expression analysis of a Toll-like receptor 22 (tlr22) gene from turbot, Scophthalmus maximus".FISH & SHELLFISH IMMUNOLOGY 44.2(2015):399-409. |
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