Cloning and expression analysis of a Toll-like receptor 22 (tlr22) gene from turbot, Scophthalmus maximus

doi:10.1016/j.fsi.2015.03.001

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	Cloning and expression analysis of a Toll-like receptor 22 (tlr22) gene from turbot, Scophthalmus maximus
	Hu, Guo-Bin 1,2; Zhang, Shou-Feng 1; Yang, Xi 1; Liu, Da-Hai 3; Liu, Qiu-Ming 1; Zhang, Shi-Cui 1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015-06
卷号	44 期号:2 页码:399-409
关键词	Scophthalmus maximus Tlr22 Structural characteristics Gene expression PAMPs
ISSN号	1050-4648
DOI	10.1016/j.fsi.2015.03.001
英文摘要	Toll-like receptor 22 (TLR22) exists exclusively in aquatic animals and recognizes double stranded RNA (dsRNA). In the present study, a tlr22 gene and its 5'-flanking sequence were cloned from turbot, Scophthalmus maximus, its immune responsive expression was subsequently studied in vivo. The turbot (sm)tlr22 gene spans over 5.6 kb with a structure of 4 exon-3 intron and encodes 962 amino acids. The deduced protein shows the highest sequence identity (76.7%) to Japanese flounder Tlr22 and possesses a signal peptide sequence, a leucine-rich repeat (LRR) domain composed of 27 LRR motifs, a transmembrane region and a Toll/interleukin-1 receptor (TIR) domain. Phylogenetic analysis grouped it with other teleost Tlr22s. The interferon-stimulated response element (ISRE) and signal transducer and activator of transcription (STAT) binding site important for the basal transcriptional activity of TLR3 were predicted in the 5'-flanking sequence of smtlr22 gene. Quantitative real-time PCR (qPCR) analysis demonstrated the constitutive expression of smtlr22 mRNA in all examined tissues with higher levels in the head kidney, kidney and spleen. Further, smtlr22 expression was significantly up-regulated following challenge with polyinosinic: polycytidylic acid (poly I:C), lipopolysaccharide (LPS) or turbot reddish body iridovirus (TRBIV) in the gills, head kidney, spleen and muscle, with maximum increases ranging from 2.56 to 6.24 fold upon different immunostimulants and organs. These findings suggest a possible role of Smtlr22 in the immune responses to the infections of a broad range of pathogens that include DNA and RNA viruses and Gram-negative bacteria. (c) 2015 Elsevier Ltd. All rights reserved.
资助项目	Shandong Provincial Natural Science Foundation[ZR2013CM045]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000354583800002
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3660]
专题	业务部门_海洋政策研究中心
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China; 2.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Peoples R China; 3.State Ocean Adm China, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Hu, Guo-Bin,Zhang, Shou-Feng,Yang, Xi,et al. Cloning and expression analysis of a Toll-like receptor 22 (tlr22) gene from turbot, Scophthalmus maximus[J]. FISH & SHELLFISH IMMUNOLOGY,2015,44(2):399-409.
APA	Hu, Guo-Bin,Zhang, Shou-Feng,Yang, Xi,Liu, Da-Hai,Liu, Qiu-Ming,&Zhang, Shi-Cui.(2015).Cloning and expression analysis of a Toll-like receptor 22 (tlr22) gene from turbot, Scophthalmus maximus.FISH & SHELLFISH IMMUNOLOGY,44(2),399-409.
MLA	Hu, Guo-Bin,et al."Cloning and expression analysis of a Toll-like receptor 22 (tlr22) gene from turbot, Scophthalmus maximus".FISH & SHELLFISH IMMUNOLOGY 44.2(2015):399-409.