Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro

doi:10.3892/br.2013.202

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro
	Pang, Min 1; Qu, Pei 2; Gao, Chun-Lei 1; Tang, Xuexi 2; Wang, Zong-Ling 1
刊名	BIOMEDICAL REPORTS
	2014
卷号	2 期号:1 页码:93-96
关键词	yessotoxin toxicity Bel7402 human hepatocellular carcinoma cells Ca2+ level
ISSN号	2049-9434
DOI	10.3892/br.2013.202
英文摘要	Yessotoxin (YTX) and its analogs are a type of marine toxins found in marine environments in numerous coastal countries. These toxins tend to accumulate in filter-feeding molluscs and may threaten the shellfish industry and public health. Several previous studies indicated that YTX may induce apoptosis in different types of cell lines, although the exact underlying mechanisms have not yet been elucidated. The aim of this study was to mainly focus on the effect of YTX on cytosolic Ca2+ levels in human hepatocellular carcinoma cells. In order to investigate the exact mechanism of YTX-evoked Ca2+ increase, laser scanning confocal microscopy was used, with the addition of the chelator ethylene glycol tetraacetic acid (EGTA) and nifedipine, an L-type Ca2+ channel blocker, to the reaction system. The results demonstrated that YTX caused cytosolic Ca2+ level increase in Bel7402 cells and the YTX-evoked Ca2+ increase was successfully blocked by EGTA and nifedipine. Therefore, our results indicated that YTX may cause apoptosis via inducing Ca2+ entry in Bel7402 cells.
资助项目	project of the Key Laboratory for Ecological Environment in Coastal Areas, SOA[201106]
WOS研究方向	Research & Experimental Medicine
语种	英语
出版者	SPANDIDOS PUBL LTD
WOS记录号	WOS:000218819000018
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/21501]
专题	业务部门_海洋生态研究中心
通讯作者	Pang, Min
作者单位	1.State Ocean Adm, Inst Oceanog 1, 6 Xianxialing Rd, Qingdao 266061, Shandong, Peoples R China 2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266100, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Pang, Min,Qu, Pei,Gao, Chun-Lei,et al. Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro[J]. BIOMEDICAL REPORTS,2014,2(1):93-96.
APA	Pang, Min,Qu, Pei,Gao, Chun-Lei,Tang, Xuexi,&Wang, Zong-Ling.(2014).Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro.BIOMEDICAL REPORTS,2(1),93-96.
MLA	Pang, Min,et al."Effect of yessotoxin on cytosolic calcium levels in human hepatocellular carcinoma cells in vitro".BIOMEDICAL REPORTS 2.1(2014):93-96.